Phenotypes associated with this allele

• Allele Symbol: Cand2^{tm1a(EUCOMM)Wtsi}
• Allele Name: targeted mutation 1a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4432020
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cand2^tm1a(EUCOMM)Wtsi/Cand2^tm1a(EUCOMM)Wtsi	C57BL/6N-Cand2^tm1a(EUCOMM)Wtsi/Wtsi	MGI:5608394
hm2	Cand2^tm1a(EUCOMM)Wtsi/Cand2^tm1a(EUCOMM)Wtsi	involves: C57BL/6N	MGI:8276487

Genotype
MGI:5608394

hm1

• Allelic Composition: Cand2^{tm1a(EUCOMM)Wtsi}/Cand2^{tm1a(EUCOMM)Wtsi}
• Genetic Background: C57BL/6N-Cand2^{tm1a(EUCOMM)Wtsi}/Wtsi
• Cell Lines: EPD0169_1_A01

Data Sources

IMPC Data for Cand2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

decreased total body fat amount ( J:211773 )

♂

IMPC - WTSI

growth/size/body

increased lean body mass ( J:211773 )

♂

IMPC - WTSI

hematopoietic system

increased red blood cell distribution width ( J:211773 )

♂

IMPC - WTSI

homeostasis/metabolism

decreased circulating thyroxine level ( J:211773 )

♂

IMPC - WTSI

decreased circulating iron level ( J:211773 )

♂

IMPC - WTSI

Genotype
MGI:8276487

hm2

• Allelic Composition: Cand2^{tm1a(EUCOMM)Wtsi}/Cand2^{tm1a(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6N
• Cell Lines: EPD0169_1_A01

cardiovascular system

decreased response of heart to induced stress ( J:374127 )

♂ • at 2 weeks after transverse aortic constriction (TAC) surgery, mice show preserved cardiac function with a significantly higher ejection fraction (EF) and fractional shortening (FS) and a significantly lower left ventricle weight to body weight (LV/BW) ratio than TAC-operated wild-type controls

♂ • at 4 weeks after TAC, both EF and FS are significantly higher while mRNA levels of Nppa and Nppb (fetal stress markers0 are 1.9-fold and 3-fold lower, respectively, than in TAC-operated wild-type controls, indicating protection against pathological remodeling in response to pressure overload

♂ • 4 weeks after TAC, mice show a ~2-fold decrease in GRK5 protein levels but no change in Grk5 mRNA levels in the left ventricles (LVs); GRK5 protein levels are blocked in nuclear but not in cytoplasmic fractions from LV lysates

♂ • pathological remodeling is decreased as early as 2 days after surgery: TAC-induced Nppa and Nppb mRNA levels and induction of MEF2A (myocyte enhancer factor 2A) target genes Xirp1 and Nr4a1 are significantly decreased; LV/BW ratio and GRK5 protein levels in LVs are significantly lower whereas protein levels of neddylated CUL1 are higher than in TAC-operated wild-type controls

♂ • however, baseline cardiac function is normal as assessed by EF and LV/BW ratio, with no significant change in Cand1, Nppa or Nppb transcript levels relative to wild-type controls

homeostasis/metabolism

decreased response of heart to induced stress ( J:374127 )

♂ • at 2 weeks after transverse aortic constriction (TAC) surgery, mice show preserved cardiac function with a significantly higher ejection fraction (EF) and fractional shortening (FS) and a significantly lower left ventricle weight to body weight (LV/BW) ratio than TAC-operated wild-type controls

♂ • at 4 weeks after TAC, both EF and FS are significantly higher while mRNA levels of Nppa and Nppb (fetal stress markers0 are 1.9-fold and 3-fold lower, respectively, than in TAC-operated wild-type controls, indicating protection against pathological remodeling in response to pressure overload

♂ • 4 weeks after TAC, mice show a ~2-fold decrease in GRK5 protein levels but no change in Grk5 mRNA levels in the left ventricles (LVs); GRK5 protein levels are blocked in nuclear but not in cytoplasmic fractions from LV lysates

♂ • pathological remodeling is decreased as early as 2 days after surgery: TAC-induced Nppa and Nppb mRNA levels and induction of MEF2A (myocyte enhancer factor 2A) target genes Xirp1 and Nr4a1 are significantly decreased; LV/BW ratio and GRK5 protein levels in LVs are significantly lower whereas protein levels of neddylated CUL1 are higher than in TAC-operated wild-type controls

♂ • however, baseline cardiac function is normal as assessed by EF and LV/BW ratio, with no significant change in Cand1, Nppa or Nppb transcript levels relative to wild-type controls