Phenotypes associated with this allele

• Allele Symbol: Ide^{tm1a(EUCOMM)Wtsi}
• Allele Name: targeted mutation 1a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4431946
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ide^tm1a(EUCOMM)Wtsi/Ide^tm1a(EUCOMM)Wtsi	B6JTyr;B6N-Ide^tm1a(EUCOMM)Wtsi/Wtsi	MGI:5781642
hm2	Ide^tm1a(EUCOMM)Wtsi/Ide^tm1a(EUCOMM)Wtsi	involves: C57BL/6N	MGI:6369650
ht3	Ide^tm1a(EUCOMM)Wtsi/Ide^+	involves: C57BL/6N	MGI:6369655

Genotype
MGI:5781642

hm1

• Allelic Composition: Ide^{tm1a(EUCOMM)Wtsi}/Ide^{tm1a(EUCOMM)Wtsi}
• Genetic Background: B6JTyr;B6N-Ide^{tm1a(EUCOMM)Wtsi}/Wtsi
• Cell Lines: EPD0158_4_G09

Data Sources

IMPC Data for Ide

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

decreased total body fat amount ( J:175295 )

IMPC - WTSI

cardiovascular system

increased heart weight ( J:175295 )

♂

IMPC - WTSI

craniofacial

abnormal cranium morphology ( J:175295 )

♂

IMPC - WTSI

abnormal incisor morphology ( J:175295 )

♂

IMPC - WTSI

abnormal maxilla morphology ( J:175295 )

♂

IMPC - WTSI

growth/size/body

increased heart weight ( J:175295 )

♂

IMPC - WTSI

abnormal incisor morphology ( J:175295 )

♂

IMPC - WTSI

decreased body weight ( J:175295 )

IMPC - WTSI

homeostasis/metabolism

decreased circulating cholesterol level ( J:175295 )

IMPC - WTSI

decreased circulating alanine transaminase level ( J:175295 )

IMPC - WTSI

increased circulating amylase level ( J:175295 )

♂

IMPC - WTSI

decreased circulating aspartate transaminase level ( J:175295 )

IMPC - WTSI

integument

abnormal hair texture ( J:175295 )

♀

IMPC - WTSI

skeleton

abnormal cranium morphology ( J:175295 )

♂

IMPC - WTSI

abnormal incisor morphology ( J:175295 )

♂

IMPC - WTSI

abnormal maxilla morphology ( J:175295 )

♂

IMPC - WTSI

Genotype
MGI:6369650

hm2

• Allelic Composition: Ide^{tm1a(EUCOMM)Wtsi}/Ide^{tm1a(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6N
• Cell Lines: EPD0158_4_G09

reproductive system

reproductive system phenotype ( J:274952 )

♂N • males show no differences in sperm motility or sperm concentration relative to wild-type males

necrospermia ( J:274952 )

♂ • sperm viability is significantly decreased (43.17 +/- 2.18%) relative to that in wild-type males (55.00 +/- 2.74%), as determined by the assessment of sperm membrane integrity using an eosin/nigrosin staining

teratozoospermia ( J:274952 )

♂ • % of abnormal sperm is significantly higher (60.00 +/- 2.73%) than that in wild-type males (43.50 +/- 2.63%)

increased testis apoptosis ( J:274952 )

♂ • mRNA expression of Bax (a proapoptotic factor) is significantly increased in testicular tissue (2.43 +/- 0.44) relative to that in wild-type males (0.57 +/- 0.25), pointing towards a proapoptotic state

♂ • however, Bcl2 and Casp3 mRNA levels remain normal

small seminiferous tubules ( J:274952 )

♂ • seminiferous tubules diameter is significantly decreased (185.56 +/- 4.71 um) relative to that in wild-type males (214.92 +/- 7.11 um)

decreased testis weight ( J:274952 )

♂ • testis weight is significantly decreased (59.75 +/- 2.59 mg) relative to that in wild-type males (121.03 +/- 1.47 mg)

♂ • gonadosomatic index (GSI = [testes weight/body weight] 100) is significantly decreased (0.44 +/- 0.06%) relative to that in wild-type males (0.73 +/- 0.03%)

cellular

necrospermia ( J:274952 )

♂ • sperm viability is significantly decreased (43.17 +/- 2.18%) relative to that in wild-type males (55.00 +/- 2.74%), as determined by the assessment of sperm membrane integrity using an eosin/nigrosin staining

teratozoospermia ( J:274952 )

♂ • % of abnormal sperm is significantly higher (60.00 +/- 2.73%) than that in wild-type males (43.50 +/- 2.63%)

increased testis apoptosis ( J:274952 )

♂ • mRNA expression of Bax (a proapoptotic factor) is significantly increased in testicular tissue (2.43 +/- 0.44) relative to that in wild-type males (0.57 +/- 0.25), pointing towards a proapoptotic state

♂ • however, Bcl2 and Casp3 mRNA levels remain normal

abnormal mitochondrial ATP synthesis coupled electron transport ( J:274952 )

♂ • mice exhibit a significant reduction in mitochondrial complex IV levels in testicular tissue (0.54 +/- 0.09) relative to wild-type males (1.00 +/- 0.05)

oxidative stress ( J:274952 )

♂ • mice exhibit increased lipid peroxidation levels in testicular tissue (1.29 +/- 0.04) relative to wild-type males (1.00 +/- 0.02), pointing towards a pro-oxidant status

♂ • however, protein nitration and carbonylation in testicular tissue is normal

endocrine/exocrine glands

increased testis apoptosis ( J:274952 )

♂ • mRNA expression of Bax (a proapoptotic factor) is significantly increased in testicular tissue (2.43 +/- 0.44) relative to that in wild-type males (0.57 +/- 0.25), pointing towards a proapoptotic state

♂ • however, Bcl2 and Casp3 mRNA levels remain normal

small seminiferous tubules ( J:274952 )

♂ • seminiferous tubules diameter is significantly decreased (185.56 +/- 4.71 um) relative to that in wild-type males (214.92 +/- 7.11 um)

decreased testis weight ( J:274952 )

♂ • testis weight is significantly decreased (59.75 +/- 2.59 mg) relative to that in wild-type males (121.03 +/- 1.47 mg)

♂ • gonadosomatic index (GSI = [testes weight/body weight] 100) is significantly decreased (0.44 +/- 0.06%) relative to that in wild-type males (0.73 +/- 0.03%)

Genotype
MGI:6369655

ht3

• Allelic Composition: Ide^{tm1a(EUCOMM)Wtsi}/Ide⁺
• Genetic Background: involves: C57BL/6N
• Cell Lines: EPD0158_4_G09

reproductive system

reproductive system phenotype ( J:274952 )

♂N • males show no significant differences in sperm motility, concentration, viability or morphology relative to wild-type males

decreased testis weight ( J:274952 )

♂ • testis weight is significantly decreased (91.75 +/- 9.38 mg) relative to that in wild-type males (121.03 +/- 1.47 mg)

♂ • gonadosomatic index (GSI = [testes weight/body weight] 100) is significantly decreased (0.54 +/- 0.04%) relative to that in wild-type males (0.73 +/- 0.03%)

endocrine/exocrine glands

decreased testis weight ( J:274952 )

♂ • testis weight is significantly decreased (91.75 +/- 9.38 mg) relative to that in wild-type males (121.03 +/- 1.47 mg)

♂ • gonadosomatic index (GSI = [testes weight/body weight] 100) is significantly decreased (0.54 +/- 0.04%) relative to that in wild-type males (0.73 +/- 0.03%)