All Phenotypes Plin5<tm1e(KOMP)Wtsi> MGI Mouse

decreased cardiac muscle triglyceride level ( J:221539 )

• 52% reduction in cardiac triacylglycerol content, along with a marked reduction of lipid droplets stained by ORO in the heart

decreased cardiac muscle triglyceride level ( J:221539 )

• 52% reduction in cardiac triacylglycerol content, along with a marked reduction of lipid droplets stained by ORO in the heart

decreased fatty acid oxidation ( J:221539 )

• ~11% decrease in whole-body fatty acid oxidation

• however, no significant alterations in fatty acid uptake, fatty acid oxidation or esterification of extracellular-derived fatty acids in isolated skeletal (soleus) muscle under basal or forskolin-stimulated conditions

increased fatty acid oxidation ( J:221539 )

• pulse-chase experiments on primary myotubes from skeletal muscle show that oxidation of triacylglycerol-derived fatty acids is increased concomitant with increased depletion of the 14C labeled triacylglycerol, indicating degradation of triacylglycerol in skeletal muscle

• however, mitochondrial content and function are not enhanced in either isolated myotubes or intact skeletal muscle

increased respiratory quotient ( J:221539 )

• respiratory exchange ratio is increased, as assessed by indirect calorimetry

improved glucose tolerance ( J:221539 )

• glucose tolerance is improved on a low-fat diet (LFD), as shown by an attenuated blood glucose excursion after glucose administration

• glucose tolerance is markedly enhanced on a high-fat diet (HFD), with HFD-fed mice showing comparable glucose tolerance to LFD-fed wild-type mice

decreased skeletal muscle glycogen level ( J:221539 )

• skeletal muscle glycogen content is reduced following hyperinsulinemic euglycemic clamp

increased insulin sensitivity ( J:221539 )

• both hepatic glucose production and insulin sensitive gluconeogenic gene expression are reduced during insulin clamp conditions, suggesting improved hepatic insulin sensitivity

insulin resistance ( J:221539 )

• mice become insulin resistant due to reduced insulin stimulated glucose disposal in skeletal muscle and adipose tissue, but maintain insulin sensitivity in the liver

• reduced skeletal muscle insulin sensitivity, observed during hyperinsulinemic euglycemic clamps, is associated with ceramide accumulation

abnormal sphingomyelin level ( J:221539 )

• sphingomyelin levels are increased in cultured myotubes

abnormal ceramide level ( J:221539 )

• ceramide level is increased by 53% in skeletal muscle, whereas diacylglycerol levels remains normal

• ceramide, dihydro- and hexosyl-ceramide levels are increased in cultured myotubes, whereas other lipid types such as phospholipids and sterols are mostly normal

• however, no evidence of ER stress, inflammation or oxidative stress in skeletal muscle

decreased skeletal muscle triglyceride level ( J:221539 )

• significant reduction of triacylglycerol content in red quadriceps (oxidative) skeletal muscle

• however, no significant alterations in mixed quadriceps or liver triacylglycerol content

abnormal carbohydrate metabolism ( J:221539 )

• ~11% increase in whole-body carbohydrate oxidation

• however, no significant alteration in glucose oxidation in isolated skeletal (soleus) muscle

enhanced lipolysis ( J:221539 )

• 24 h fasted mice fail to show lipid droplet staining in skeletal muscle, indicating increased intramyocelluar lipolysis and an inability to expand the intramyocellular triacylglycerol pool

decreased cardiac muscle triglyceride level ( J:221539 )

• 52% reduction in cardiac triacylglycerol content, along with a marked reduction of lipid droplets stained by ORO in the heart

decreased skeletal muscle glycogen level ( J:221539 )

• skeletal muscle glycogen content is reduced following hyperinsulinemic euglycemic clamp

decreased skeletal muscle triglyceride level ( J:221539 )

• significant reduction of triacylglycerol content in red quadriceps (oxidative) skeletal muscle

• however, no significant alterations in mixed quadriceps or liver triacylglycerol content

decreased adipocyte glucose uptake ( J:221539 )

• glucose uptake is reduced in white adipose tissue

decreased adipocyte glucose uptake ( J:221539 )

• glucose uptake is reduced in white adipose tissue

decreased fatty acid oxidation ( J:221539 )

• ~11% decrease in whole-body fatty acid oxidation

• however, no significant alterations in fatty acid uptake, fatty acid oxidation or esterification of extracellular-derived fatty acids in isolated skeletal (soleus) muscle under basal or forskolin-stimulated conditions

increased fatty acid oxidation ( J:221539 )

• pulse-chase experiments on primary myotubes from skeletal muscle show that oxidation of triacylglycerol-derived fatty acids is increased concomitant with increased depletion of the 14C labeled triacylglycerol, indicating degradation of triacylglycerol in skeletal muscle

• however, mitochondrial content and function are not enhanced in either isolated myotubes or intact skeletal muscle

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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