Phenotypes associated with this allele

• Allele Symbol: Pdx1^tm1.1Stof
• Allele Name: targeted mutation 1.1, Doris Stoffers
• Allele ID: MGI:4359453
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pdx1^tm1.1Stof/Pdx1^tm1.1Stof	involves: 129/Sv * C57BL/6	MGI:4359482
ht2	Pdx1^tm1.1Stof/Pdx1^+	involves: 129/Sv * C57BL/6	MGI:4359483
ht3	Pdx1^tm1Cvw/Pdx1^tm1.1Stof	involves: 129/Sv * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:4359484

Genotype
MGI:4359482

hm1

• Allelic Composition: Pdx1^tm1.1Stof/Pdx1^tm1.1Stof
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Reduction in Ngn3+ pancreatic endocrine progenitor cells in Pdx1^tm1.1Stof/Pdx1⁺ and Pdx1^tm1.1Stof/Pdx1^tm1.1Stof mice

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:152582 )

N • pancreatic weight and appearance are normal

abnormal PP cell morphology ( J:152582 )

• at E17.5, PP cells occupy less pancreatic area than in wild-type mice

decreased pancreatic alpha cell mass ( J:152582 )

• at E17.5, alpha cells occupy less pancreatic area than in wild-type mice

decreased pancreatic beta cell mass ( J:152582 )

• at E17.5, beta cells occupy less pancreatic area than in wild-type mice

abnormal pancreatic delta cell morphology ( J:152582 )

• at E17.5, delta cells occupy less pancreatic area than in wild-type mice

abnormal pancreatic epsilon cell morphology ( J:152582 )

• at E17.5, epsilon cells occupy less pancreatic area than in wild-type mice

pancreatic islet hypoplasia ( J:152582 )

• at E17.5, endocrine lineage cells are reduced compared to in wild-type mice with beta and alpha cells most severely affected

abnormal pancreas development ( J:152582 )

• from E13.5, Ngn3+ endocrine progenitor cells are decreased in number and staining intensity compared to in wild-type mice

• at E19.5, apoptosis of Ngn3+ endocrine progenitor cells is increased compared to in wild-type mice

• however, the number of Ngn3+ endocrine progenitor cells at E11.5 is normal and progenitor cell proliferation is normal

homeostasis/metabolism

hyperglycemia ( J:152582 )

• hypoglycemia is mild at birth but progressively worsens with age

decreased circulating insulin level ( J:152582 )

• in fasting mice or following glucose stimulation

impaired glucose tolerance ( J:152582 )

• at 3 to 4 weeks, mice are diabetic unlike wild-type mice

Genotype
MGI:4359483

ht2

• Allelic Composition: Pdx1^tm1.1Stof/Pdx1⁺
• Genetic Background: involves: 129/Sv * C57BL/6

Reduction in Ngn3+ pancreatic endocrine progenitor cells in Pdx1^tm1.1Stof/Pdx1⁺ and Pdx1^tm1.1Stof/Pdx1^tm1.1Stof mice

endocrine/exocrine glands

decreased pancreatic beta cell mass ( J:152582 )

• at E17.5, beta cells occupy less pancreatic area than in wild-type mice

pancreas hypoplasia ( J:152582 )

abnormal pancreatic endocrine progenitor cell physiology ( J:152582 )

• at E19.5, apoptosis of Ngn3+ endocrine progenitor cells is increased compared to in wild-type mice

• however, the number of Ngn3+ endocrine progenitor cells at E17.5 is normal

homeostasis/metabolism

decreased circulating insulin level ( J:152582 )

• unlike similarly treated wild-type mice, glucose-stimulated male mice fail to exhibit an increase in plasma insulin levels while female mice exhibit reduced fasting insulin levels

impaired glucose tolerance ( J:152582 )

• at 3 to 4 weeks, male mice are glucose intolerant unlike wild-type mice

• however, female mice exhibit normal glucose tolerance at 3 to 4 weeks of age

Genotype
MGI:4359484

ht3

• Allelic Composition: Pdx1^tm1Cvw/Pdx1^tm1.1Stof
• Genetic Background: involves: 129/Sv * 129S1/Sv * 129X1/SvJ * C57BL/6

Pancreatic hypoplasia in Pdx1^tm1Cvw/Pdx1^tm1.1Stof mice

endocrine/exocrine glands

small pancreas ( J:152582 )

• small compared to in wild-type mice but not as small as in Pdx1^tm1Cvw homozygotes

decreased pancreas weight ( J:152582 )

• decreased weight compared to in wild-type mice but not as much as in Pdx1^tm1Cvw homozygotes

homeostasis/metabolism

hyperglycemia ( J:152582 )

• at birth