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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Rnf8Gt(AS0574)Wtsi
gene trap AS0574, Wellcome Trust Sanger Institute
MGI:4346727
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Rnf8Gt(AS0574)Wtsi/Rnf8Gt(AS0574)Wtsi involves: 129P2/OlaHsd * C57BL/6 MGI:4459433
ht2
 		Rnf8Gt(AS0574)Wtsi/Rnf8+ involves: 129P2/OlaHsd * C57BL/6 MGI:4459435


Genotype
MGI:4459433
hm1
Allelic
Composition		 Rnf8Gt(AS0574)Wtsi/Rnf8Gt(AS0574)Wtsi
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rnf8Gt(AS0574)Wtsi mutation (0 available); any Rnf8 mutation (19 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Impaired spermatogenesis in Rnf8Gt(AS0574)Wtsi/Rnf8Gt(AS0574)Wtsi and Rnf8Gt(RRR260)Byg/Rnf8Gt(RRR260)Byg males

mortality/aging
increased mortality induced by gamma-irradiation ( J:161240 )
• only 10% of mice treated with 8 Gy of gamma radiation are viable compared with 60% of similarly treated wild-type mice
premature death ( J:161240 )
• only 56% of mice are viable at 465 days compared with 97% of wild-type mice
• some mice severely runted mice die prematurely without signs of tumors

reproductive system
abnormal spermatogonia morphology ( J:161240 )
• few spermatogonia are found within the seminiferous epithelium unlike in wild-type mice
abnormal seminiferous tubule epithelium morphology ( J:161240 )
• disorganized
• few spermatogonia are found within the seminiferous epithelium unlike in wild-type mice
seminiferous tubule degeneration ( J:161240 )
• in severely affected mice without inflammation
abnormal spermatogenesis ( J:161240 )
• at 1.5 to 3.5 months of age
azoospermia ( J:161240 )
• seminifrous tubules few to no mature spermatozoa unlike in wild-type mice
oligozoospermia ( J:161240 )
• seminifrous tubules few to no mature spermatozoa unlike in wild-type mice
decreased litter size ( J:161240 )
• from male mice

immune system
increased thymocyte apoptosis ( J:161240 )
• thymocytes exhibit increased radiosensitivity compared with similarly treated wild-type cells
abnormal class switch recombination ( J:161240 )
• class switch recombination is impaired compared to in wild-type mice

cellular
azoospermia ( J:161240 )
• seminifrous tubules few to no mature spermatozoa unlike in wild-type mice
oligozoospermia ( J:161240 )
• seminifrous tubules few to no mature spermatozoa unlike in wild-type mice
abnormal spermatogonia morphology ( J:161240 )
• few spermatogonia are found within the seminiferous epithelium unlike in wild-type mice
increased cellular sensitivity to gamma-irradiation ( J:161240 )
• bone marrow cells exhibit increased sensitivity to gamma irradiation compared with similarly treated wild-type cells
increased thymocyte apoptosis ( J:161240 )
• thymocytes exhibit increased radiosensitivity compared with similarly treated wild-type cells
decreased fibroblast proliferation ( J:161240 )
• mouse embryonic fibroblasts exhibit decreased cell proliferation compared with wild-type cells
• however, proliferation of mouse embryonic fibroblasts is rescued by hypoxic conditions
induced chromosome breakage ( J:161240 )
• following irradiation, B cells exhibit chromosome structural aberrations compared with similarly treated wild-type cells
spontaneous chromosome breakage ( J:161240 )
• chromosome breaks and metaphase aberrations in activated B cells are increased 4.8-fold compared to in similarly treated wild-type cells

neoplasm

growth/size/body
decreased body size ( J:161240 )
decreased body weight ( J:161240 )
postnatal growth retardation ( J:161240 )

endocrine/exocrine glands
increased thymocyte apoptosis ( J:161240 )
• thymocytes exhibit increased radiosensitivity compared with similarly treated wild-type cells
abnormal seminiferous tubule epithelium morphology ( J:161240 )
• disorganized
• few spermatogonia are found within the seminiferous epithelium unlike in wild-type mice
seminiferous tubule degeneration ( J:161240 )
• in severely affected mice without inflammation

hematopoietic system
increased thymocyte apoptosis ( J:161240 )
• thymocytes exhibit increased radiosensitivity compared with similarly treated wild-type cells
abnormal class switch recombination ( J:161240 )
• class switch recombination is impaired compared to in wild-type mice

integument

homeostasis/metabolism
increased mortality induced by gamma-irradiation ( J:161240 )
• only 10% of mice treated with 8 Gy of gamma radiation are viable compared with 60% of similarly treated wild-type mice




Genotype
MGI:4459435
ht2
Allelic
Composition		 Rnf8Gt(AS0574)Wtsi/Rnf8+
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rnf8Gt(AS0574)Wtsi mutation (0 available); any Rnf8 mutation (19 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:161240 )
• only 77% of mice are viable at 465 days compared with 97% of wild-type mice
• some mice severely runted mice die prematurely without signs of tumors

immune system
abnormal class switch recombination ( J:161240 )
• class switch recombination is impaired compared to in wild-type mice but not as severely as in homozygous mice

cellular
increased cellular sensitivity to gamma-irradiation ( J:161240 )
• bone marrow cells treated with 4 Gy or 6 Gy exhibit a modest sensitivity compared with similarly treated wild-type cells

growth/size/body
decreased body size ( J:161240 )

neoplasm
neoplasm ( J:161240 )
N
• tumorigenesis is not significantly different than in wild-type mice

hematopoietic system
abnormal class switch recombination ( J:161240 )
• class switch recombination is impaired compared to in wild-type mice but not as severely as in homozygous mice




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04/30/2024
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