Analysis Tools
mortality/aging
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• no homozygous null offspring were found beyond E7.5 from heterozygous cross
• homozygous embryos died after implantation and before E10.5, a time period during which gastrulation and early organogenesis occurs
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embryo
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• embryos were unusually dense in appearance, shorter, and characterized by a dumb-bell shape at E 6.5
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• clearly defined extra-embryonic- and embryonic ectoderm-derived epithelial structures were lost by E 8
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• the region of the epiblast undergoing EMT transition appeared broader
• mutant epiblast was actively undergoing EMT and that the loose differentiated mesoderm are abundant
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• despite the absence of a morphological node, the patterning of the most anterior and posterior mesoderm are not blocked, but rather affected the abundance of these cells
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• The ectoplacental cone of mutants easily separated from the embryo.
• The embryonic ectoderm was present but thicker in structure and not organized into regular epithelia.
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• clearly defined extra-embryonic- and embryonic ectoderm-derived epithelial structures were lost by E 8
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• Reichert's membrane failed to expand, and remained tightly associated with the visceral endoderm.
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• The extraembryonic ectoderm was disorganized and separated from the ectoplacental cone by a constriction of visceral and parietal endoderm.
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• no morphological evidence for primitive erythroid cells
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• definitive endoderm marker positive cells fail to displace the visceral endoderm
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cardiovascular system
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• abundant throughout all but the most distal region
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hematopoietic system
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• no morphological evidence for primitive erythroid cells
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homeostasis/metabolism
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• induced teratomas from E 7.5 embryos with differentiated tissues comparable to the wild type control but are smaller in size
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cellular
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• abundant throughout all but the most distal region
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