Phenotypes associated with this allele

• Allele Symbol: Nrbf2^{Gt(Ayu21-W143)Imeg}
• Allele Name: gene trap Ayu21-W143, Institute of Molecular Embryology and Genetics
• Allele ID: MGI:3923820
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nrbf2^Gt(Ayu21-W143)Imeg/Nrbf2^Gt(Ayu21-W143)Imeg	involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj	MGI:6380656

Genotype
MGI:6380656

hm1

• Allelic Composition: Nrbf2^{Gt(Ayu21-W143)Imeg}/Nrbf2^{Gt(Ayu21-W143)Imeg}
• Genetic Background: involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

increased hepatocyte apoptosis ( J:220500 )

• livers exhibit sporadic apoptosis of hepatocytes

abnormal liver morphology ( J:220500 )

• mice exhibit focal ductular reaction indicative of liver injury by 10 months of age

focal hepatic necrosis ( J:220500 )

• mice develop focal liver necrosis by 10 months of age

homeostasis/metabolism

abnormal autophagosome formation ( J:220500 )

• MEFs show a decrease of autophagosome numbers under normal conditions and rapamycin treatment to induced autophagy

• MEFs also show a decrease in phagophores, the autophagosome precursors under normal condition, rapamycin treatment, and nutrient withdrawal

• marker analysis indicates impaired autophagosome maturation, particularly under autophagy induction

impaired autophagy ( J:220500 )

• mice show partially impaired autophagy in livers

cellular

abnormal autophagosome formation ( J:220500 )

• MEFs show a decrease of autophagosome numbers under normal conditions and rapamycin treatment to induced autophagy

• MEFs also show a decrease in phagophores, the autophagosome precursors under normal condition, rapamycin treatment, and nutrient withdrawal

• marker analysis indicates impaired autophagosome maturation, particularly under autophagy induction

impaired autophagy ( J:220500 )

• mice show partially impaired autophagy in livers

increased sensitivity to induced cell death ( J:220500 )

• MEFs are more susceptible to ER stress induced by thapsigargin or tunicamycin, with cells showing impaired autophagy activation and an increase in morphology changes and cell death

increased hepatocyte apoptosis ( J:220500 )

• livers exhibit sporadic apoptosis of hepatocytes

focal hepatic necrosis ( J:220500 )

• mice develop focal liver necrosis by 10 months of age

mortality/aging

mortality/aging ( J:220500 )

N • mice show no enhanced mortality compared to wild-type mice