Phenotypes associated with this allele

• Allele Symbol: Cdc20^tm1.1Pzg
• Allele Name: targeted mutation 1.1, Pumin Zhang
• Allele ID: MGI:3851133
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cdc20^tm1.1Pzg/Cdc20^tm1.1Pzg	involves: 129S7/SvEvBrd * C57BL/6	MGI:3851136
ht2	Cdc20^tm1.1Pzg/Cdc20^+	involves: 129S7/SvEvBrd * C57BL/6	MGI:3851137

Genotype
MGI:3851136

hm1

• Allelic Composition: Cdc20^tm1.1Pzg/Cdc20^tm1.1Pzg
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Aberrant mitoses in Cdc20^tm1.1Pzg/Cdc20^tm1.1Pzg MEFs

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:150421 )

• die around E12.5

cellular

aneuploidy ( J:150421 )

• in cultured MEFs as early as passage 3 the percentage of cells with abnormal karyotypes in increased compared to wild-type cells

abnormal mitosis ( J:150421 )

• in MEFs, in metaphase chromosomes are misaligned and in anaphase lagging chromosomes or chromosome bridges are seen

• anaphase begins prematurely

abnormal cell death ( J:150421 )

• cell growth in cultured MEFs stops with increasing passage numbers as the result of massive cell death

• cell death can be rescued by immortalizing MEFs with the expression of the SV40 large T antigen

increased embryonic tissue cell apoptosis ( J:150421 )

• very high levels of apoptotic cell death are seen in embryos

decreased cell proliferation ( J:150421 )

• MEFs grow more slowly than controls

embryo

increased embryonic tissue cell apoptosis ( J:150421 )

• very high levels of apoptotic cell death are seen in embryos

decreased embryo size ( J:150421 )

growth/size/body

decreased embryo size ( J:150421 )

Genotype
MGI:3851137

ht2

• Allelic Composition: Cdc20^tm1.1Pzg/Cdc20⁺
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

Liver tumor of a Cdc20^tm1.1Pzg/Cdc20⁺ mouse

cellular

aneuploidy ( J:150421 )

• in cultured MEFs as early as passage 3 and in splenocytes from adult mice the percentage of cells with abnormal karyotypes in increased compared to wild-type controls

neoplasm

increased tumor incidence ( J:150421 )

• palpable tumors can be detected by 6.8 months of age and by 24 months half of mutants have tumors compared to only 10% of wild-type controls

• of mice with tumors, 38% have tumors at multiple organ sites

increased hepatoma incidence ( J:150421 )

• 4 of 29 tumors were hepatomas

increased lymphoma incidence ( J:150421 )

• 25 of 29 tumors were lymphomas

liver/biliary system

increased hepatoma incidence ( J:150421 )

• 4 of 29 tumors were hepatomas