mortality/aging
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• while present at E3.5, no embryos are detected at E6.5
• embryos fail to develop beyond the morula stage
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embryo
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• morulas fail to undergo compaction and apoptose
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• at E3.5, embryos have not progressed beyond the morula stage and fail to do so when cultured unlike wild-type embryos
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cellular
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• E3.5 cells fail to form a mature, tripartite nucleoli unlike wild-type cells
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Analysis Tools