Phenotypes associated with this allele

• Allele Symbol: Hcst^tm1.1Dnax
• Allele Name: targeted mutation 1.1, DNAX Research Institute of Cellular and Molecular Biology
• Allele ID: MGI:3843505
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hcst^tm1.1Dnax/Hcst^tm1.1Dnax	involves: C57BL/6	MGI:3843519

Genotype
MGI:3843519

hm1

• Allelic Composition: Hcst^tm1.1Dnax/Hcst^tm1.1Dnax
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:148086 )

• mice injected with RMA RAE-1delta cells exhibit increased mortality compared to similarly treated wild-type mice

• however, mortality in response to injection with RMA cells is normal

immune system

immune system phenotype ( J:148086 )

N • mice do not exhibit any spontaneous autoimmune phenotype

increased spleen weight ( J:148086 )

impaired natural killer cell mediated cytotoxicity ( J:148086 )

• lysis of tumor cells is impaired compared to in wild-type mice

abnormal NK T cell physiology ( J:147867 , J:148086 )

• NK cells infected with mouse cytomegalovirus undergo less proliferation than in similarly treated wild-type cells (J:147867)

• NK T cells are hyperactive and exhibit increased proliferation in the spleen and bone marrow compared to in wild-type mice (J:148086)

decreased interferon-gamma secretion ( J:147867 )

• antibody cross-linking of the Klra8 (Ly49H) receptor in cultured NK cells induces reduced IFNgamma secretion compared to in similarly treated wild-type cells

• however, normal amounts of IFNgamma are produced following stimulation by the NK1.1 receptor

increased interferon-gamma secretion ( J:148086 )

• TCR-stimulated NK T cells produce more IFNgamma than similarly treated wild-type cells

increased interleukin-4 secretion ( J:148086 )

• in culture, NK T cells activated with anti-CD3 and anti-NK1.1 antibodies exhibit increased IL4 production compared to similarly treated wild-type cells

• TCR-stimulated NK T cells produce more IL4 than similarly treated wild-type cells

increased susceptibility to experimental autoimmune encephalomyelitis ( J:148086 )

• 40% MOG-treated mice develop experimental autoimmune encephalomyelitis compared to none of the similarly treated wild-type mice

abnormal response to infection ( J:147867 )

• 1 week after infection with mouse cytomegalovirus, viral titers persist in the salivary glands unlike in wild-type mice

• however, viral clearance from the spleen and liver is normal

neoplasm

decreased incidence of tumors by chemical induction ( J:148086 )

• DMBA-treated mice develop fewer tumors than similarly treated wild-type mice

decreased metastatic potential ( J:148086 )

• mice injected with B16 melanoma cells exhibit 3 to 5 times fewer pulmonary metastasis compared to similarly treated wild-type mice

• however, antibody depletion of NKT cells or treatment with anti-CD25 antibodies prior to tumor cell injection restores metastatic potential

decreased tumor growth/size ( J:148086 )

• DMBA-T tumors cells exhibit reduced growth compared to when they are transplanted into wild-type mice

• however, antibody-based depletion of NK and NKT cells or treatment with GM1 antibodies restores normal growth rate of cancer cells

homeostasis/metabolism

ascites ( J:148086 )

• after 2 weeks after injection with RMA or RMA RAE-1delta cells, 20% of mice develop ascites whereas mice injected with RMA RAE-1delta cells do not

• most mice develop ascites 3 weeks after injection with RMA or RMA RAE-1delta cells whereas mice injected with RMA RAE-1delta cells do not

decreased incidence of tumors by chemical induction ( J:148086 )

• DMBA-treated mice develop fewer tumors than similarly treated wild-type mice

hematopoietic system

increased spleen weight ( J:148086 )

impaired natural killer cell mediated cytotoxicity ( J:148086 )

• lysis of tumor cells is impaired compared to in wild-type mice

abnormal NK T cell physiology ( J:147867 , J:148086 )

• NK cells infected with mouse cytomegalovirus undergo less proliferation than in similarly treated wild-type cells (J:147867)

• NK T cells are hyperactive and exhibit increased proliferation in the spleen and bone marrow compared to in wild-type mice (J:148086)

growth/size/body

increased spleen weight ( J:148086 )