Phenotypes associated with this allele

• Allele Symbol: E2f3^tm2Lees
• Allele Name: targeted mutation 2, Jacqueline A Lees
• Allele ID: MGI:3840180
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	E2f3^tm2Lees/E2f3^tm2Lees	either: 129S/Sv-E2f3^tm2Lees or (involves: 129S4/SvJae * C57BL/6)	MGI:3840225
cx2	E2f1^tm1Njd/E2f1^tm1Njd E2f3^tm2Lees/E2f3^tm2Lees	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:3840227

Genotype
MGI:3840225

hm1

• Allelic Composition: E2f3^tm2Lees/E2f3^tm2Lees
• Genetic Background: either: 129S/Sv-E2f3^tm2Lees or (involves: 129S4/SvJae * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell physiology ( J:147270 )

• MEFs in culture sometimes grow more slowly

abnormal cell cycle ( J:147270 )

• MEFs in culture sometimes show defects in cell cycle re-entry

Genotype
MGI:3840227

cx2

• Allelic Composition: E2f1^tm1Njd/E2f1^tm1Njd; E2f3^tm2Lees/E2f3^tm2Lees
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6

mortality/aging

neonatal lethality, incomplete penetrance ( J:147270 )

• significantly reduced survival to weaning

• normal numbers at birth

• most mortality is in the neonatal period

growth/size/body

decreased body weight ( J:147270 )

• double homozygotes weight about 1/3 of controls

skeleton

abnormal cartilage morphology ( J:147270 )

• mature cartilage present

abnormal long bone epiphyseal plate morphology ( J:147270 )

• chondrocytes disorganized

• stacked structures are not seen in femurs

abnormal chondrocyte morphology ( J:147270 )

• abnormal morphology

• 1.5-2 fold larger than normal in resting, columnar, and hypertrophic zones

• increased variability in size

cellular

abnormal cell cycle ( J:147270 )

• MEFs in culture sometimes display impaired re-entry into the cell cycle

decreased cell proliferation ( J:147270 )

• impaired asynchronous proliferation of MEFs in culture