Phenotypes associated with this allele

• Allele Symbol: Glud1^tm1.1Pma
• Allele Name: targeted mutation 1.1, Pierre Maechler
• Allele ID: MGI:3835667
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Glud1^tm1.1Pma/Glud1^tm1.1Pma	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:3835755
cn2	Glud1^tm1.1Pma/Glud1^tm1.1Pma Tg(Ins2-cre)23Herr/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA/J * SJL	MGI:3835754

Genotype
MGI:3835755

cn1

• Allelic Composition: Glud1^tm1.1Pma/Glud1^tm1.1Pma
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased insulin secretion ( J:145558 )

• cre-adenovirus treated islet cells exhibit a 38% reduction in glucose-stimulated insulin secretion compared to similarly treated wild-type cells

• cre-adenovirus treated islet cells exhibit a 47% reduction in glutamine plus L-leucine analogue BCH-stimulated insulin secretion compared to similarly treated wild-type cells

endocrine/exocrine glands

decreased insulin secretion ( J:145558 )

• cre-adenovirus treated islet cells exhibit a 38% reduction in glucose-stimulated insulin secretion compared to similarly treated wild-type cells

• cre-adenovirus treated islet cells exhibit a 47% reduction in glutamine plus L-leucine analogue BCH-stimulated insulin secretion compared to similarly treated wild-type cells

Genotype
MGI:3835754

cn2

• Allelic Composition: Glud1^tm1.1Pma/Glud1^tm1.1Pma; Tg(Ins2-cre)23Herr/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA/J * SJL

Glud1^tm1.1Pma/Glud1^tm1.1Pma Tg(Ins2-cre)23Herr/0 mice have impaired insulin secretion

endocrine/exocrine glands

disorganized pancreatic islets ( J:145558 )

• 67% of islets are disorganized compared to 30% in wild-type mice

• however, pancreas insulin content is normal

decreased insulin secretion ( J:145558 )

• glucose-stimulated insulin secretion in the pancreas is decreased 48% during the first phase and 37% in the second phase compared to in similarly treated wild-type mice

• isolated islets exhibit a 68% reduction in the first phase and 49% reduction in the second phase of glucose-stimulated insulin secretion compared to similarly treated wild-type islets

• insulin secretion is decreased 38% 15 minutes following intraperitoneal glucose injection at 2 months of age

• however, fasted insulin levels are normal and ectopic expression of Glud1 in islet cells restores normal glucose-stimulated insulin secretion

growth/size/body

growth/size/body region phenotype ( J:145558 )

N • despite disruptions in stimulated insulin secretion, mice exhibit normal growth

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:145558 )

N • despite disruptions in stimulated insulin secretion, mice exhibit normal glucose homeostasis and age-dependent insulin resistance

decreased insulin secretion ( J:145558 )

• glucose-stimulated insulin secretion in the pancreas is decreased 48% during the first phase and 37% in the second phase compared to in similarly treated wild-type mice

• isolated islets exhibit a 68% reduction in the first phase and 49% reduction in the second phase of glucose-stimulated insulin secretion compared to similarly treated wild-type islets

• insulin secretion is decreased 38% 15 minutes following intraperitoneal glucose injection at 2 months of age

• however, fasted insulin levels are normal and ectopic expression of Glud1 in islet cells restores normal glucose-stimulated insulin secretion

decreased circulating insulin level ( J:145558 )

• 55% when in a fed state

abnormal enzyme/coenzyme activity ( J:145558 )

• glutamate dehydrogenase activity is reduced by 60% in islets compared to in wild-type mice