Phenotypes associated with this allele

• Allele Symbol: Batf3^tm1Kmm
• Allele Name: targeted mutation 1, Kenneth M Murphy
• Allele ID: MGI:3817232
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Batf3^tm1Kmm/Batf3^tm1Kmm	involves: 129S6/SvEvTac	MGI:5313304
hm2	Batf3^tm1Kmm/Batf3^tm1Kmm	involves: 129S6/SvEvTac * BALB/cJ	MGI:3817249
cx3	Batf3^tm1Kmm/Batf3^tm1Kmm Id2^tm1Gtbz/Id2^tm1Gtbz	B6.Cg-Batf3^tm1Kmm Id2^tm1Gtbz	MGI:5486201

Genotype
MGI:5313304

hm1

• Allelic Composition: Batf3^tm1Kmm/Batf3^tm1Kmm
• Genetic Background: involves: 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:181366 )

N • homozygotes retain an enhanced antibody response to anti-Clec9a coupled antigen

abnormal cytotoxic T cell physiology ( J:181366 )

• reduced cytotoxic T cell response to anti-Clec9a coupled antigen

abnormal dendritic cell antigen presentation ( J:362930 )

• mice immunized with OVA-beads + poly(I:C) do not mount a strong OVA-specific CD8+ T cell response

• mice immunized with ultraviolet-irradiated, OVA-coated NIH-3T3 cells (H-2^q) + poly(I:C) show a reduced OVA-specific CD8+ T cell response

hematopoietic system

abnormal cytotoxic T cell physiology ( J:181366 )

• reduced cytotoxic T cell response to anti-Clec9a coupled antigen

Genotype
MGI:3817249

hm2

• Allelic Composition: Batf3^tm1Kmm/Batf3^tm1Kmm
• Genetic Background: involves: 129S6/SvEvTac * BALB/cJ

immune system

abnormal dendritic cell differentiation ( J:141064 )

• mice lack CD8a⁺ dendritic cell subsets

• bone marrow chimera experiments indicate a lack of CD8a⁺ DCs is due to a cell-intrinsic effect of the mutant locus

• in vitro bone marrow differentiation cultures fail to generate CD24⁺ Sirp-alpha^- DCs that are similar to CD8a⁺ DC

decreased myeloid dendritic cell number ( J:141064 )

• mice lack splenic CD11c^hiCD8a⁺DEC205⁺ dendritic cells

• mice have a loss of CD11c^hiCD11b^dull DCs and CD11c^hi CD8a⁺CD24⁺ DCs

• mice have normal populations of CD4⁺ and CD8a-CD4- conventional DC subsets

• lymph nodes and thymi lack CD8a⁺ DC subsets

• CD103-⁺DEC205⁺CD8a^-CD11b^lo/- dermal DC numbers are reduced 5-fold in draining lymph nodes of the skin

decreased memory T cell number ( J:141064 )

• mice fail to develop West Nile Virus (WNV)-specific memory CD8⁺ T cells after infection with the virus

• the number of CD8⁺CD44^hiCD62L^lowcells memory cells is about half-that of wild-type mice before infection and is five-fold less after infection

abnormal immune system physiology ( J:141064 )

• transplanted syngenic fibrosarcomas grow in size instead of being reapidly rejected as happens in control mice

abnormal cytotoxic T cell physiology ( J:141064 )

• CD8⁺ T cells from mice infected with West Nile virus (WNV) have defective IFN-gamma secretion when cultured with WNV-peptide

• bone marrow chimera studies demonstrate that defective cytolytic activity is not intrinsic to the CD8⁺ T cells but rather defective dendritic cell function

• infiltrating CD8⁺ T cells fail to infiltrate transplanted syngenic tumors

decreased cytotoxic T cell cytolysis ( J:141064 )

• CD8⁺ T cells from mice infected with West Nile virus (WNV) have defective killing of WNV peptide-loaded target cells

abnormal dendritic cell physiology ( J:141064 )

• conventional DCs fail to secrete IL-12 after TL3 stimulation but have normal responses to TLR4 and TLR9 stimulation

abnormal dendritic cell antigen presentation ( J:141064 )

• conventional DCs are unable to cross-present (i.e. take up protein, process into peptide and present) ovalbumin antigen to ova-specific CD8⁺ T cells

hematopoietic system

abnormal dendritic cell differentiation ( J:141064 )

• mice lack CD8a⁺ dendritic cell subsets

• bone marrow chimera experiments indicate a lack of CD8a⁺ DCs is due to a cell-intrinsic effect of the mutant locus

• in vitro bone marrow differentiation cultures fail to generate CD24⁺ Sirp-alpha^- DCs that are similar to CD8a⁺ DC

decreased myeloid dendritic cell number ( J:141064 )

• mice lack splenic CD11c^hiCD8a⁺DEC205⁺ dendritic cells

• mice have a loss of CD11c^hiCD11b^dull DCs and CD11c^hi CD8a⁺CD24⁺ DCs

• mice have normal populations of CD4⁺ and CD8a-CD4- conventional DC subsets

• lymph nodes and thymi lack CD8a⁺ DC subsets

• CD103-⁺DEC205⁺CD8a^-CD11b^lo/- dermal DC numbers are reduced 5-fold in draining lymph nodes of the skin

decreased memory T cell number ( J:141064 )

• mice fail to develop West Nile Virus (WNV)-specific memory CD8⁺ T cells after infection with the virus

• the number of CD8⁺CD44^hiCD62L^lowcells memory cells is about half-that of wild-type mice before infection and is five-fold less after infection

abnormal cytotoxic T cell physiology ( J:141064 )

• CD8⁺ T cells from mice infected with West Nile virus (WNV) have defective IFN-gamma secretion when cultured with WNV-peptide

• bone marrow chimera studies demonstrate that defective cytolytic activity is not intrinsic to the CD8⁺ T cells but rather defective dendritic cell function

• infiltrating CD8⁺ T cells fail to infiltrate transplanted syngenic tumors

decreased cytotoxic T cell cytolysis ( J:141064 )

• CD8⁺ T cells from mice infected with West Nile virus (WNV) have defective killing of WNV peptide-loaded target cells

cellular

abnormal dendritic cell differentiation ( J:141064 )

• mice lack CD8a⁺ dendritic cell subsets

• bone marrow chimera experiments indicate a lack of CD8a⁺ DCs is due to a cell-intrinsic effect of the mutant locus

• in vitro bone marrow differentiation cultures fail to generate CD24⁺ Sirp-alpha^- DCs that are similar to CD8a⁺ DC

Genotype
MGI:5486201

cx3

• Allelic Composition: Batf3^tm1Kmm/Batf3^tm1Kmm; Id2^tm1Gtbz/Id2^tm1Gtbz
• Genetic Background: B6.Cg-Batf3^tm1Kmm Id2^tm1Gtbz

immune system

abnormal dendritic cell physiology ( J:194760 )

• in reconstitution assays, CD8alpha+ dendritic cells generate from transplanted bone marrow cells fail to survive long-term

• CD8alpha+ dendritic cells exhibit impaired cell-associated cross-presentation compared with control cells

• however, cells exhibit normal soluble cross-presentation and cell-associated antigen cross-presentation during GVHD or HSV-1 viral antigens