All Phenotypes Ifit1<tm1(KOMP)Vlcg> MGI Mouse

abnormal T cell physiology ( J:205203 )

• MHV-A59-infected mice show enhanced T cell stimulation

abnormal interferon level ( J:205203 )

• MHV-A59-infected mice show reduced interferon-alpha4 and interferon-beta1 mRNA levels in the brain and spinal cord at 3 and 5 dpi and IFN levels in brain are reduced at 5 dpi

• MHV-A59-infected mice show increased IFN-gamma levels

• interferon-alpha/beta mRNA levels are reduced more severely microglia and macrophages of MHV-A59-infected mice

brain inflammation ( J:205203 )

• MHV-A59-infected mice show rapid progression of encephalitis symptoms with nearly 60% having severe encephalitis

increased susceptibility to Coronaviridae infection ( J:205203 )

• mice intracranially infected with mouse hepatitis virus MHV-A59 show earlier onset of disease and more severe morbidity, with clinical disease as early as 4 days post infection (dpi)

• MHV-A59-infected mice that show a low clinical score by 5 dpi recover by 14 dpi, despite higher disease severity than wild-type mice during the recovery phase

• MHV-A59-infected mice show increased infectious virus in the brain and spinal cord by 5 dpi and increased viral levels in the liver

• however, no evidence of hepatitis is seen in MHV-A59-infected mice

• MHV-A59 viral antigen is increased throughout the brain, including the cerebellum which shows minimal infection in wild-type mice

• microglia is the most frequently infected cell type but mice show a higher number of infected neurons in the cerebrum and brainstem

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:205203 )

• 50-60% of MHV-A59-infected mice show mortality between 6 and 8 dpi compared to 100% survival in wild-type mice

• mice infected with a 5-fold increase in MHV-A59 virus dose show 90% mortality compared to 100% survival in wild-type mice