Phenotypes associated with this allele

• Allele Symbol: Kcnk15^{tm1(KOMP)Vlcg}
• Allele Name: targeted mutation 1, Velocigene
• Allele ID: MGI:3808238
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kcnk15^tm1(KOMP)Vlcg/Kcnk15^tm1(KOMP)Vlcg	C57BL/6N-Kcnk15^tm1(KOMP)Vlcg	MGI:7786390

Genotype
MGI:7786390

hm1

• Allelic Composition: Kcnk15^{tm1(KOMP)Vlcg}/Kcnk15^{tm1(KOMP)Vlcg}
• Genetic Background: C57BL/6N-Kcnk15^{tm1(KOMP)Vlcg}
• Cell Lines: 10341A-F11

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal nervous system electrophysiology ( J:359116 )

• in response to prolonged depolarization, phasically firing neurons (mostly bushy cells) of the ventral cochlear nucleus (VCN) exhibit a significantly higher rheobase current than wild-type controls, whereas the rheobase currents of tonically firing VCN neurons (most likely stellate cells) and of phasically firing principal neurons of the medial nucleus of the trapezoid body (MNTB) are not significantly altered

• in response to step depolarizations from -100 mV to +40 mV from a holding voltage of -70 mV, both VCN neurons and MNTB principal neurons show larger leak currents than wild-type controls, with a less pronounced effect in MNTB cells

• phasically firing VCN neurons show a significantly lower input resistance (R_I) whereas MNTB principal cells show a slightly higher R_I than wild-type controls, indicating changes in the number of ion channels open at rest

• tonically firing VCN neurons show normal R_I relative to wild-type controls

abnormal action potential ( J:359116 )

• tonically firing VCN neurons exhibit a slightly increased number of action potentials (APs) in response to a given current injection relative to wild-type controls, resulting in a steeper input/output curve but with no significant changes at individual current strengths

• in response to short depolarizations, both types of VCN neurons as well as phasically firing MNTB principal neurons show normal AP amplitude, resting membrane potential, and AP width relative to wild-type controls

• phasically firing VCN neurons show no significant change in the number of APs fired in response to a given current injection

hearing/vestibular/ear

abnormal auditory brainstem response ( J:359116 )

• 6- to 8 weeks-old mice show mild alterations in pure tone ABR thresholds, with the largest change detected in the high frequency domain (32 kHz)

• however, ABR thresholds in response to click and noise stimulation are normal

abnormal auditory brainstem response waveform shape ( J:359116 )

• amplitude of wave III (=superior olivary complex) is significantly decreased upon application of noise stimuli at high SPLs, with no significant reduction noted in response to click stimuli

• in contrast to the amplitude reductions in waves I and III, the amplitude of wave IV (= combined activity of the lateral lemniscus and the inferior colliculus) is significantly increased only in response to click stimuli

• analysis of click- and noise-induced ABR waves showed reduced amplitudes of wave I (=auditory nerve fibers) at high sound pressure levels (SPLs) for both types of stimuli, whereas the amplitude of wave II (=cochlear nucleus) is unaffected independent of the stimulus presented