Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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N |
• the presence of the transgene prevents the partial arrest of B cell development that occurs in Igk-Ctm1Jkbv homozygotes
• normal numbers of B cells are found at all developmental stages including immature and mature stages
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• 75% of mature B cells express human kappa light chain
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• levels of antibody containing human kappa light chain are 1,400 micrograms per ml while levels of antibody containing murine lambda light chain are 100 micrograms per ml
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hematopoietic system
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• 75% of mature B cells express human kappa light chain
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• levels of antibody containing human kappa light chain are 1,400 micrograms per ml while levels of antibody containing murine lambda light chain are 100 micrograms per ml
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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N |
• the presence of the transgene prevents the partial arrest of B cell development that occurs in Igk-Ctm1Jkbv homozygotes
• normal numbers of B cells are found at all developmental stages including immature and mature stages
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• over 90% of mature B cells express human kappa light chain
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hematopoietic system
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• over 90% of mature B cells express human kappa light chain
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH1)2BJkbv mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
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• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
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• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
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• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
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• pre-B cells numbers in the bone marrow are 70% of wild-type
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hematopoietic system
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
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• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
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• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
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• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
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• pre-B cells numbers in the bone marrow are 70% of wild-type
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2)J9.2Jkbv mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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N |
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
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• the levels of immature B cells found in circulation are 70% that found in wild-type mice
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively
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hematopoietic system
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• the levels of immature B cells found in circulation are 70% that found in wild-type mice
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2BM)7Amgn mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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• mice have 60-80% the number of B cells as wild-type mice
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• unimmunized mice produce significant levels of human IgM and IgG1
• IgM levels in sera ranged from 110-140 micrograms/ml
• IgG1 levels in sera ranged from 172-298 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences
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hematopoietic system
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• mice have 60-80% the number of B cells as wild-type mice
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• unimmunized mice produce significant levels of human IgM and IgG1
• IgM levels in sera ranged from 110-140 micrograms/ml
• IgG1 levels in sera ranged from 172-298 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2CM)2Amgn mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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• mice have 80-100% the number of B cells as wild-type mice
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• unimmunized mice produce significant levels of human IgM and IgG2
• IgM levels in sera ranged from 27.3-109.4 micrograms/ml
• IgG2 levels in sera ranged from 37.6-665.3 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences
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hematopoietic system
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• mice have 80-100% the number of B cells as wild-type mice
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• unimmunized mice produce significant levels of human IgM and IgG2
• IgM levels in sera ranged from 27.3-109.4 micrograms/ml
• IgG2 levels in sera ranged from 37.6-665.3 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yHG1/2)1Amgn mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
|
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immune system
|
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• immunizing mice with human antigens leads to production of antigen-specific IgG1 antibodies with human sequences
|
hematopoietic system
|
|
• immunizing mice with human antigens leads to production of antigen-specific IgG1 antibodies with human sequences
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yHG4)1Amgn mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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• immunizing mice with human antigens leads to production of antigen-specific IgG4 antibodies with human sequences
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hematopoietic system
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• immunizing mice with human antigens leads to production of antigen-specific IgG4 antibodies with human sequences
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|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2)J9.2Jkbv mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
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• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
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• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
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• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
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• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen
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hematopoietic system
|
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• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
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|
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• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
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• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
|
|
|
• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
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• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen
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Analysis Tools