Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Tg(IGH@yH2)J9.2Jkbv mutation
(0 available)
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immune system
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N |
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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• antibodies containing human mu or gamma2 heavy chains are found in the sera at levels of 200 micrograms per ml
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hematopoietic system
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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• antibodies containing human mu or gamma2 heavy chains are found in the sera at levels of 200 micrograms per ml
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2)J9.2Jkbv mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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N |
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
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• the levels of immature B cells found in circulation are 70% that found in wild-type mice
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively
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hematopoietic system
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• the levels of immature B cells found in circulation are 70% that found in wild-type mice
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
|
|
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• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively
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|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2)J9.2Jkbv mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
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immune system
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• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
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• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
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• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
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• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
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• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen
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hematopoietic system
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• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
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• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
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• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
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• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
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• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen
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Analysis Tools