Analysis Tools
mortality/aging
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• only 17% of mice at weaning are homozygotes instead of the expected 25%
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• expected proportion of homozygotes is found at E14.5 but not at weaning
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adipose tissue
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• appear leaner
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• average cell diameter is about 61% of wild-type and total DNA content is only 56% of wild-type indicating fewer cells are present
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• at 12 weeks of age the gonadal fat pad is 38% the size in control littermates
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• at 12 weeks of age the retroperitoneal fat pad is 15% the size in control littermates
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cellular
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• following exposure to inducers of adipocyte differentiation, MEFs display enhanced lipid accumulation
• however, cell cycle and proliferation are similar to wild-type
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growth/size/body
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• similar in size at birth but significantly smaller than littermate controls from 3 to 12 weeks of age
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