Phenotypes associated with this allele

• Allele Symbol: Gli3^tm1.1Alj
• Allele Name: targeted mutation 1.1, Alexandra L Joyner
• Allele ID: MGI:3798850
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gli3^tm1.1Alj/Gli3^tm1.1Alj	involves: 129S6/SvEvTac * C57BL/6	MGI:3803094
ht2	Gli3^tm1.1Alj/Gli3^Xt	involves: 101/H * 129S6/SvEvTac * C3H/HeH * C57BL/6 * CBA/H	MGI:3803095
cx3	Fgf8^tm1.4Mrt/Fgf8^+ Gli3^tm1.1Alj/Gli3^tm1.1Alj	involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6	MGI:3803097

Genotype
MGI:3803094

hm1

• Allelic Composition: Gli3^tm1.1Alj/Gli3^tm1.1Alj
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal midbrain-hindbrain boundary morphology ( J:137136 )

• loss of diagnostic morphology of isthmus

• anterior isthmus region with characteristics of the cerebellum

• thicker than normal Purkinje region and axons disorganized

• dorsal mesencephalon, isthmus, and r1 not distinct at E10.5 and E12.5

• isthmus flexure less prominent

abnormal midbrain morphology ( J:137136 )

• tegmentum unaffected

• ventral nuclei containing dopaminergic neurons appear normal

enlarged cerebral aqueduct ( J:137136 )

• expanded mesencephalic ventricle at E10.5 and E12.5

abnormal inferior colliculus morphology ( J:137136 )

• poorly formed

• loss of diagnostic morphology

abnormal superior colliculus morphology ( J:137136 )

• loss of diagnostic morphology

enlarged tectum ( J:137136 )

• overgrown tectum

abnormal hindbrain morphology ( J:137136 )

• thickness of ventricular zone increased

abnormal cerebellum morphology ( J:137136 )

• not clearly separated from the isthmus at E18.5

reduced cerebellar foliation ( J:137136 )

• poorly foliated

abnormal cerebellar Purkinje cell layer ( J:137136 )

• thicker than normal

• axons disorganized in lateral regions

Genotype
MGI:3803095

ht2

• Allelic Composition: Gli3^tm1.1Alj/Gli3^Xt
• Genetic Background: involves: 101/H * 129S6/SvEvTac * C3H/HeH * C57BL/6 * CBA/H

nervous system

abnormal rhombomere morphology ( J:137136 )

• loss of diagnostic morphology of isthmus

• anterior isthmus region with characteristics of the cerebellum

• thicker than normal Purkinje region and axons disorganized

• dorsal mesencephalon, isthmus, and r1 not distinct at E10.5 and E12.5

• isthmus flexure less prominent

abnormal midbrain morphology ( J:137136 )

• tegmentum unaffected

• ventral nuclei containing dopaminergic neurons appear normal

enlarged cerebral aqueduct ( J:137136 )

• expanded mesencephalic ventricle at E10.5 and E12.5

abnormal inferior colliculus morphology ( J:137136 )

• poorly formed

• loss of diagnostic morphology

abnormal superior colliculus morphology ( J:137136 )

• loss of diagnostic morphology

enlarged tectum ( J:137136 )

• overgrown tectum

abnormal hindbrain morphology ( J:137136 )

• thickness of ventricular zone increased

abnormal cerebellum morphology ( J:137136 )

• not clearly separated from the isthmus at E18.5

reduced cerebellar foliation ( J:137136 )

• poorly foliated

abnormal cerebellar Purkinje cell layer ( J:137136 )

• thicker than normal

• axons disorganized in lateral regions

embryo

abnormal rhombomere morphology ( J:137136 )

• loss of diagnostic morphology of isthmus

• anterior isthmus region with characteristics of the cerebellum

• thicker than normal Purkinje region and axons disorganized

• dorsal mesencephalon, isthmus, and r1 not distinct at E10.5 and E12.5

• isthmus flexure less prominent

Genotype
MGI:3803097

cx3

• Allelic Composition: Fgf8^tm1.4Mrt/Fgf8⁺; Gli3^tm1.1Alj/Gli3^tm1.1Alj
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6

nervous system

nervous system phenotype ( J:137136 )

N • cerebellum has begun to foliate at birth

N • granule layer extends along the anterior posterior length of the cerebellum

N • isthmus is formed

N • Purkinje cells are normal

absent inferior colliculus ( J:137136 )

• not clearly distinguishable

enlarged tectum ( J:137136 )