Phenotypes associated with this allele

• Allele Symbol: Slc18a3^tm1Mca
• Allele Name: targeted mutation 1, Marc G Caron
• Allele ID: MGI:3798809
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Slc18a3^tm1Mca/Slc18a3^tm1Mca	involves: 129S4/SvJae * C57BL/6J	MGI:3803092
ht2	Slc18a3^tm1Mca/Slc18a3^+	involves: 129S4/SvJae * C57BL/6J	MGI:3803093

Genotype
MGI:3803092

hm1

• Allelic Composition: Slc18a3^tm1Mca/Slc18a3^tm1Mca
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:136620 )

• following treatment with pilocarpine, mice exhibit a shorter latency to the first epileptiform discharge and first seizure episode compared to wild-type mice

• however, duration of seizure episodes was not different than in similarly treated wild-type mice

impaired coordination ( J:137284 )

• in a wire-hang and rotarod test

decreased grip strength ( J:137284 )

• grip strength is impaired

• however, grip strength can be improved by prior injections of cholinesterase inhibitors

abnormal social investigation ( J:137284 )

• mice exhibit a deficit in social recognition memory compared to wild-type mice

impaired exercise endurance ( J:137284 )

• mice are unable to maintain long periods of physical activities unlike wild-type mice

nervous system

increased susceptibility to pharmacologically induced seizures ( J:136620 )

• following treatment with pilocarpine, mice exhibit a shorter latency to the first epileptiform discharge and first seizure episode compared to wild-type mice

• however, duration of seizure episodes was not different than in similarly treated wild-type mice

abnormal endplate potential ( J:137284 )

• steady-state depression of evoked endplate potential is greater than in wild-type mice

abnormal miniature endplate potential ( J:137284 )

• mice exhibit smaller and less frequent miniature endplate potentials than in wild-type mice

abnormal synaptic acetylcholine release ( J:137284 )

• acetylcholine release is decreased compared to in wild-type mice

• acetylcholine levels in the brain are increased compared to in wild-type and heterozygous mice due to reduced release

homeostasis/metabolism

impaired exercise endurance ( J:137284 )

• mice are unable to maintain long periods of physical activities unlike wild-type mice

Genotype
MGI:3803093

ht2

• Allelic Composition: Slc18a3^tm1Mca/Slc18a3⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

behavior/neurological

behavior/neurological phenotype ( J:137284 )

N • despite increased social investigation, mice perform normally in hidden food reward tests and test order habituation indicating normal olfactory function

abnormal object recognition memory ( J:137284 )

• mice exhibit decreased ability to remember familiar objects compared to wild-type mice

impaired coordination ( J:137284 )

• mice exhibit impaired performance in a rotarod test only after 12 trials

abnormal social investigation ( J:137284 )

• mice fail to exhibit a decrease in exploration of an intruder mouse upon re-exposure until the fourth re-exposure unlike wild-type mice

• in a social versus non-social stimuli test, mice exhibit a preference for non-social stimuli unlike wild-type mice

• however, treatment of mice by prior injections of cholinesterase inhibitors restores normal socialization to repeated exposure to an intruder mouse

nervous system

abnormal miniature endplate potential ( J:137284 )

• smaller than in wild-type mice

abnormal synaptic acetylcholine release ( J:137284 )

• extracellular acetylcholine levels are depressed more than 35% in the frontal cortex and about 31% in the striatum compared to wild-type mice

• KCL stimulated release of acetylcholine is reduced relative to in wild-type mice

• acetylcholine levels in the brain are increased 49% compared to in wild-type mice due to reduced release