Phenotypes associated with this allele

• Allele Symbol: Cplx3^tm1Bros
• Allele Name: targeted mutation 1, Nils Brose
• Allele ID: MGI:3796622
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cplx3^tm1Bros/Cplx3^tm1Bros	Not Specified	MGI:3807186
cx2	Cplx3^tm1Bros/Cplx3^tm1Bros Cplx4^tm1Bros/Cplx4^tm1Bros	involves: C57BL/6	MGI:3851183
cx3	Cplx1^tm2Bros/Cplx1^tm2Bros Cplx2^tm1Bros/Cplx2^tm1Bros Cplx3^tm1Bros/Cplx3^tm1Bros	Not Specified	MGI:3807187
cx4	Cplx2^tm1Bros/Cplx2^tm1Bros Cplx3^tm1Bros/Cplx3^tm1Bros	Not Specified	MGI:3807188

Genotype
MGI:3807186

hm1

• Allelic Composition: Cplx3^tm1Bros/Cplx3^tm1Bros
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:136393 )

N • mice are viable

vision/eye

vision/eye phenotype ( J:150490 )

N • despite eye electrophysiological abnormalities, no defects in retinal morphology are detected

abnormal eye electrophysiology ( J:150490 )

• peak power and peak frequency of oscillatory potentials are reduced

abnormal cone electrophysiology ( J:150490 )

• prolongation of the implicit time for the b wave especially at higher light intensities

nervous system

nervous system phenotype ( J:136393 )

N • action potential-evoked excitatory and inhibitory postsynaptic currents are normal

N • despite alterations in synaptic transmission, readily releasable pools are normal

reproductive system

reproductive system phenotype ( J:136393 )

N • mice are fertile

Genotype
MGI:3851183

cx2

• Allelic Composition: Cplx3^tm1Bros/Cplx3^tm1Bros; Cplx4^tm1Bros/Cplx4^tm1Bros
• Genetic Background: involves: C57BL/6

vision/eye

abnormal retina photoreceptor morphology ( J:150490 )

• many photoreceptor ribbon synapse complexes are disintegrated

• in adults photoreceptor terminals containing spherical free floating ribbons are seen

disorganized retina outer plexiform layer ( J:150490 )

abnormal eye electrophysiology ( J:150490 )

• significant reduction in b-wave amplitudes and prolongation of b-wave implicit times at all light intensities

• slight increase in a-wave amplitudes and prolongation of a-wave implicit times at medium light intensities

• peak power but not peak frequency of oscillatory potentials is reduced

abnormal vision ( J:150490 )

• mice take more time to reach the visible platform in a cued platform test in the Morris water maze; however, mice show a normal learning curve in this test and normal motor function indicating the delay is due to impaired vision

• in contrast, performance in a visual cliff test is similar to controls

nervous system

abnormal retina photoreceptor morphology ( J:150490 )

• many photoreceptor ribbon synapse complexes are disintegrated

• in adults photoreceptor terminals containing spherical free floating ribbons are seen

Genotype
MGI:3807187

cx3

• Allelic Composition: Cplx1^tm2Bros/Cplx1^tm2Bros; Cplx2^tm1Bros/Cplx2^tm1Bros; Cplx3^tm1Bros/Cplx3^tm1Bros
• Genetic Background: Not Specified

mortality/aging

neonatal lethality, complete penetrance ( J:136393 )

• mice die within a few hours of birth

nervous system

abnormal pre-Botzinger complex physiology ( J:136393 )

• the amplitude and frequency of spontaneous excitatory postsynaptic currents are decreased in pre-Botzinger complex neurons compared to in wild-type mice

• spontaneous mEPSC frequencies are decreased in hippocampal neurons and pre-Botzinger complex neurons compared to in wild-type

abnormal neuron physiology ( J:136393 )

• neuron calcium sensitivity of glutamate and GABA release is reduced compared to in wild-type mice

impaired synaptic plasticity ( J:136393 )

• trains of action potentials produce a marked initial facilitation compared to a the depression observed in wild-type mice

decreased excitatory postsynaptic current amplitude ( J:136393 )

• evoked and spontaneous excitatory postsynaptic current amplitudes in hippocampal neurons are reduced compared to in wild-type mice

• in pre-Botzinger complex neurons compared to in wild-type mice

decreased excitatory postsynaptic current frequency ( J:136393 )

• in pre-Botzinger complex neurons compared to in wild-type mice

abnormal inhibitory postsynaptic currents ( J:136393 )

• mice exhibit a reduced evoked inhibitory postsynaptic currents and slightly reduced miniature inhibitory postsynaptic currents compared to wild-type mice

• spontaneous miniature inhibitory postsynaptic current frequencies are decreased in hippocampal and pre-Botzinger complex neurons compared to in wild-type mice

abnormal miniature excitatory postsynaptic currents ( J:136393 )

• evoked miniature excitatory postsynaptic currents (mEPSCs) are slightly reduced while spontaneous mEPSCs are reduced 30% compared to in wild-type mice

• spontaneous mEPSC frequencies are decreased in hippocampal neurons and pre-Botzinger complex neurons compared to in wild-type mice

• the amplitude of spontaneous mEPSC in hippocampal neurons is decreased compared to in wild-type mice

abnormal neurotransmitter secretion ( J:136393 )

• the probability of vesicular release of the readily releasable pool is reduced 50% compared to in wild-type mice

• neuron calcium sensitivity of glutamate and GABA release is reduced compared to in wild-type mice

• however, the size of the readily releasable pool is normal

Genotype
MGI:3807188

cx4

• Allelic Composition: Cplx2^tm1Bros/Cplx2^tm1Bros; Cplx3^tm1Bros/Cplx3^tm1Bros
• Genetic Background: Not Specified

mortality/aging

mortality/aging ( J:136393 )

N • mice are viable

nervous system

nervous system phenotype ( J:136393 )

N • action potential-evoked excitatory and inhibitory postsynaptic currents are normal

N • despite alterations in synaptic transmission, readily releasable pools are normal

reproductive system

reproductive system phenotype ( J:136393 )

N • mice are fertile