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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Stim2tm1Rao
targeted mutation 1, Anjana Rao
MGI:3778913
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Stim2tm1Rao/Stim2tm1Rao
Tg(Cd4-cre)1Cwi/0 		involves: C57BL/6 * DBA/2 * SJL MGI:3783459
cn2
 		Stim1tm1Rao/Stim1tm1Rao
Stim2tm1Rao/Stim2tm1Rao
Tg(Cd4-cre)1Cwi/0 		involves: C57BL/6 * DBA/2 * SJL MGI:3783460


Genotype
MGI:3783459
cn1
Allelic
Composition		 Stim2tm1Rao/Stim2tm1Rao
Tg(Cd4-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * DBA/2 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stim2tm1Rao mutation (1 available); any Stim2 mutation (42 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal CD4-positive, alpha-beta T cell physiology ( J:133262 )
• CD4+ T cells produce much less IL-2 and interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells
• however, calcium flux within CD4+ T cells are relatively normal
abnormal T-helper 1 physiology ( J:133262 )
• T helper type 1 and 2 cells produce less IL-2, IL-4 and interferon-gamma compared to wild-type cells
abnormal T-helper 2 physiology ( J:133262 )
• T helper type 1 and 2 cells produce less IL-2, IL-4 and interferon-gamma compared to wild-type cells
decreased interferon-gamma secretion ( J:133262 )
• CD4+ T cells produce much less interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells
• T helper type 1 and 2 cells produce less IL-2, IL-4 and interferon-gamma compared to wild-type cells
decreased interleukin-2 secretion ( J:133262 )
• CD4+ T cells produce much less IL-2 after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells
• T helper type 1 and 2 cells produce less IL-2, IL-4 and interferon-gamma compared to wild-type cells
decreased interleukin-4 secretion ( J:133262 )
• T helper type 1 and 2 cells produce less IL-2, IL-4 and interferon-gamma compared to wild-type cells

hematopoietic system
abnormal CD4-positive, alpha-beta T cell physiology ( J:133262 )
• CD4+ T cells produce much less IL-2 and interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells
• however, calcium flux within CD4+ T cells are relatively normal
abnormal T-helper 1 physiology ( J:133262 )
• T helper type 1 and 2 cells produce less IL-2, IL-4 and interferon-gamma compared to wild-type cells
abnormal T-helper 2 physiology ( J:133262 )
• T helper type 1 and 2 cells produce less IL-2, IL-4 and interferon-gamma compared to wild-type cells




Genotype
MGI:3783460
cn2
Allelic
Composition		 Stim1tm1Rao/Stim1tm1Rao
Stim2tm1Rao/Stim2tm1Rao
Tg(Cd4-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * DBA/2 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stim1tm1Rao mutation (1 available); any Stim1 mutation (46 available)
Stim2tm1Rao mutation (1 available); any Stim2 mutation (42 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:133262 )
• mice produce more IgE+ B cells than wild-type mice
abnormal T cell differentiation ( J:133262 )
• mice produce more memory or effector T cells than wild-type mice
• as determined by bone marrow transplant assays, mice exhibit a cell-intrinsic defect in regulatory T cell development
abnormal granulocyte morphology ( J:133262 )
• mice produce more CD11b+ IL-5R+ eosinophils or basophils than wild-type mice
decreased regulatory T cell number ( J:133262 )
• at 5 to 6 weeks of age, mice exhibit fewer regulatory T cells in the thymus, spleen and lymph nodes compared to wild-type mice due to a cell-intrinsic defect in regulatory T cell development
• while the proportion of regulatory T cells increases with age it remains 10% to 20% of wild-type
enlarged spleen ( J:133262 )
• in mice older than 8 weeks
enlarged lymph nodes ( J:133262 )
• in mice older than 8 weeks
decreased T cell proliferation ( J:133262 )
• T cells undergo reduced proliferation compared to wild-type cells after TCR stimulation
abnormal CD4-positive, alpha-beta T cell physiology ( J:133262 )
• CD4+ T cells exhibit no calcium influx after depletion with thapsigargin or stimulation with anti-CD3 antibodies and ionomycin unlike wild-type cells
• CD4+ T cells produce almost no IL-2 and very little interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells
decreased interferon-gamma secretion ( J:133262 )
• CD4+ T cells produce very little interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells
decreased interleukin-2 secretion ( J:133262 )
• CD4+ T cells produce almost no IL-2 after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells
blepharitis ( J:133262 )
• in mice older than 8 weeks
dermatitis ( J:133262 )
• in mice older than 8 weeks

vision/eye
blepharitis ( J:133262 )
• in mice older than 8 weeks

hematopoietic system
decreased T cell proliferation ( J:133262 )
• T cells undergo reduced proliferation compared to wild-type cells after TCR stimulation
enlarged spleen ( J:133262 )
• in mice older than 8 weeks
abnormal B cell differentiation ( J:133262 )
• mice produce more IgE+ B cells than wild-type mice
abnormal T cell differentiation ( J:133262 )
• mice produce more memory or effector T cells than wild-type mice
• as determined by bone marrow transplant assays, mice exhibit a cell-intrinsic defect in regulatory T cell development
abnormal granulocyte morphology ( J:133262 )
• mice produce more CD11b+ IL-5R+ eosinophils or basophils than wild-type mice
decreased regulatory T cell number ( J:133262 )
• at 5 to 6 weeks of age, mice exhibit fewer regulatory T cells in the thymus, spleen and lymph nodes compared to wild-type mice due to a cell-intrinsic defect in regulatory T cell development
• while the proportion of regulatory T cells increases with age it remains 10% to 20% of wild-type
abnormal CD4-positive, alpha-beta T cell physiology ( J:133262 )
• CD4+ T cells exhibit no calcium influx after depletion with thapsigargin or stimulation with anti-CD3 antibodies and ionomycin unlike wild-type cells
• CD4+ T cells produce almost no IL-2 and very little interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells

integument
dermatitis ( J:133262 )
• in mice older than 8 weeks

cellular
decreased T cell proliferation ( J:133262 )
• T cells undergo reduced proliferation compared to wild-type cells after TCR stimulation

growth/size/body
enlarged spleen ( J:133262 )
• in mice older than 8 weeks




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Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory