Phenotypes associated with this allele

• Allele Symbol: Myh10^{tm6(Myh9/GFP)Rsad}
• Allele Name: targeted mutation 6, Robert S Adelstein
• Allele ID: MGI:3775953
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Myh10^tm6(Myh9/GFP)Rsad/Myh10^tm6(Myh9/GFP)Rsad	involves: 129S6/SvEvTac * C57BL/6	MGI:3801465

Genotype
MGI:3801465

hm1

• Allelic Composition: Myh10^{tm6(Myh9/GFP)Rsad}/Myh10^{tm6(Myh9/GFP)Rsad}
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Cardiac defects during embryonic development in Myh10^{tm6(Myh9/GFP)Rsad}/Myh10^{tm6(Myh9/GFP)Rsad} mice

growth/size/body

cardiac hypertrophy ( J:124610 )

• surviving mutants exhibit higher heart/body weight ratio at 3 months of age and increased expression of hypertrophy-related genes

mortality/aging

decreased survivor rate ( J:124610 )

• only 15 of 172 F2 generation mutants survive to adulthood

lethality throughout fetal growth and development, incomplete penetrance ( J:124610 )

• most F2 generation mutants die by E16.5

• no live mutants are generated following the F2 and F3 crosses

• 15 of 172 F2 generation mutants survive to adulthood

cardiovascular system

increased myocardial fiber size ( J:124610 )

• increase in the size of cardiac myocytes at 3 months of age

abnormal cardiac outflow tract development ( J:245570 )

• at E11.5, cardiac myocytes do not invade the underlying cardiac cushions, indicating defective myocardialization of the developing outflow tract

abnormal fetal cardiomyocyte morphology ( J:124610 )

• impaired cytokinesis of cardiac myocytes, showing increased binucleation

decreased fetal cardiomyocyte number ( J:124610 )

• cardiac myocytes are decreased by 70% compared to controls at E16.5

increased fetal cardiomyocyte size ( J:124610 )

• cardiac myocytes are enlarged at E13.5

double outlet right ventricle ( J:124610 )

• 5 of 7 F3 generation mutants between E13.5 and E16.5 have dextroposition of the aorta resulting in a double outlet right ventricle (DORV)

ventricular septal defect ( J:124610 )

• 7 of 7 F3 generation mutants between E13.5 and E16.5 have a ventricular septal defect (VSD)

cardiac hypertrophy ( J:124610 )

• surviving mutants exhibit higher heart/body weight ratio at 3 months of age and increased expression of hypertrophy-related genes

cardiac interstitial fibrosis ( J:124610 )

• surviving mutants show presence of interstitial fibrosis in the heart at 3 months of age

dilated cardiomyopathy ( J:124610 )

• surviving mutants do not exhibit ventricular septal defect or double outlet right ventticle, however starting at 3 month of age, they show signs of dilated cardiomyopathy

decreased cardiac muscle contractility ( J:124610 )

• 3 month old hearts show a decrease in fractional shortening and an increase in the end systolic and diastolic dimensions

muscle

increased myocardial fiber size ( J:124610 )

• increase in the size of cardiac myocytes at 3 months of age

dilated cardiomyopathy ( J:124610 )

• surviving mutants do not exhibit ventricular septal defect or double outlet right ventticle, however starting at 3 month of age, they show signs of dilated cardiomyopathy

decreased cardiac muscle contractility ( J:124610 )

• 3 month old hearts show a decrease in fractional shortening and an increase in the end systolic and diastolic dimensions

nervous system

nervous system phenotype ( J:124610 )

N • no evidence of hydrocephalus as seen in Myh10^tm2Rsad mice

abnormal neuronal migration ( J:124610 )

• facial and pontine neurons migrate abnormally and the former protrudes into the fourth ventricle

abnormal cerebellar foliation ( J:124610 )

• minor alterations in the foliation pattern at E16.5

cellular

cardiac interstitial fibrosis ( J:124610 )

• surviving mutants show presence of interstitial fibrosis in the heart at 3 months of age

abnormal neuronal migration ( J:124610 )

• facial and pontine neurons migrate abnormally and the former protrudes into the fourth ventricle