cellular
 |
• following induction of superovulation with PMSG and hCG, the majority of mutant oocytes exhibit signs of degeneration
|
mortality/aging
|
|
• although mutant females display normal fertility and cycling at 2-3 months of age, they gradually lose fertility leading to near complete infertility by 6 months of age
|
reproductive system
|
|
• at 4 months of age, mutant females display signs of hemorrhagic ovarian cysts; however, hemorrhagic cysts are significantly fewer than those observed in Esr1tm1.1Mma females
• following injection of PMSG alone or PMSG+hCG, mutant ovaries develop multiple hemorrhagic follicles, rarely observed in wild-type ovaries
|
|
|
• following induction of superovulation with PMSG and hCG, the majority of mutant oocytes exhibit signs of degeneration
|
|
|
• at 4 months of age, mutant ovaries display interstitial hypertrophy
|
|
|
• at 4 months of age, mutant females display reduced numbers of corpora lutea relative to age-matched control females
|
|
|
• at 4 months of age, mutant females exhibit increased numbers of late preantral and early antral stage follicles
|
|
|
• following induction of superovulation with PMSG +hCG, most theca cells in mutant ovaries are abnormally located in interstitial areas, in contrast to wild-types ovaries where Cyp17-positive cells are mostly found in the theca interna of preovulatory follicles
|
|
|
• following induction of superovulation with PMSG +hCG injections, ovaries of 6-month-old mutant females are more heavily populated with theca (Cyp17-positive) cells than wild-type ovaries
• similarly, on the day of diestrus, ovaries of 4- and 10-month-old mutant females exhibit higher numbers of Cyp17-positive cells than wild-type ovaries
|
|
|
• at 4 months of age, unstimulated mutant females display signs of hemorrhagic ovarian cysts; however, hemorrhagic cysts are significantly fewer than those observed in Esr1tm1.1Mma females
|
|
|
• although mutant females display normal fertility and cycling at 2-3 months of age, they gradually lose fertility leading to near complete infertility by 6 months of age
|
|
|
• following induction of superovulation with PMSG and hCG, significantly fewer numbers of oocytes are retrieved from the oviducts of 6-mo-old mutant females than from age-matched wild-type control females (6 vs 22 oocytes, respectively)
• in contrast, only marginally smaller numbers of oocytes are retrieved at 2 months of age (20 vs 27 oocytes)
|
|
|
• at 4 months of age or later, all mutant females display an irregular pattern of estrous cycles relative to age-matched control littermates
|
|
|
• at 4- and 6 months of age, all mutant females exhibit a prolonged diestrous phase relative to age-matched control littermates
|
|
|
• female mutants become infertile before they reach 6 months of age
|
growth/size/body
|
|
• at 4 months of age, unstimulated mutant females display signs of hemorrhagic ovarian cysts; however, hemorrhagic cysts are significantly fewer than those observed in Esr1tm1.1Mma females
|
endocrine/exocrine glands
|
|
• at 4 months of age, mutant females display signs of hemorrhagic ovarian cysts; however, hemorrhagic cysts are significantly fewer than those observed in Esr1tm1.1Mma females
• following injection of PMSG alone or PMSG+hCG, mutant ovaries develop multiple hemorrhagic follicles, rarely observed in wild-type ovaries
|
|
|
• unexpectedly, at 4 months of age, mutant anterior pituitaries show a 2-fold increase in gonadotroph cell number relative to wild-type controls, with an approximately similar level of LH content in each cell
|
|
|
• at 4 months of age, mutant ovaries display interstitial hypertrophy
|
|
|
• at 4 months of age, mutant females display reduced numbers of corpora lutea relative to age-matched control females
|
|
|
• at 4 months of age, mutant females exhibit increased numbers of late preantral and early antral stage follicles
|
|
|
• following induction of superovulation with PMSG +hCG, most theca cells in mutant ovaries are abnormally located in interstitial areas, in contrast to wild-types ovaries where Cyp17-positive cells are mostly found in the theca interna of preovulatory follicles
|
|
|
• following induction of superovulation with PMSG +hCG injections, ovaries of 6-month-old mutant females are more heavily populated with theca (Cyp17-positive) cells than wild-type ovaries
• similarly, on the day of diestrus, ovaries of 4- and 10-month-old mutant females exhibit higher numbers of Cyp17-positive cells than wild-type ovaries
|
|
|
• at 4 months of age, unstimulated mutant females display signs of hemorrhagic ovarian cysts; however, hemorrhagic cysts are significantly fewer than those observed in Esr1tm1.1Mma females
|
homeostasis/metabolism
nervous system
|
|
• unexpectedly, at 4 months of age, mutant anterior pituitaries show a 2-fold increase in gonadotroph cell number relative to wild-type controls, with an approximately similar level of LH content in each cell
|
cardiovascular system
|
|
• at 4 months of age, mutant females display signs of hemorrhagic ovarian cysts; however, hemorrhagic cysts are significantly fewer than those observed in Esr1tm1.1Mma females
• following injection of PMSG alone or PMSG+hCG, mutant ovaries develop multiple hemorrhagic follicles, rarely observed in wild-type ovaries
|
Analysis Tools