Phenotypes associated with this allele

• Allele Symbol: Ldhc^tm1Erg
• Allele Name: targeted mutation 1, Erwin Goldberg
• Allele ID: MGI:3775042
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ldhc^tm1Erg/Ldhc^tm1Erg	involves: 129S6/SvEvTac * C57BL/6N	MGI:3811275

Genotype
MGI:3811275

hm1

• Allelic Composition: Ldhc^tm1Erg/Ldhc^tm1Erg
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:132072 )

N • despite impaired fertility, weights of the testis, epididymis and seminal vesicles are normal and testis morphology is similar to controls

abnormal testis physiology ( J:132072 )

♂ • lactate dehydrogenase activity is reduced by about 50% compared to wild-type controls

male infertility ( J:132072 )

♂ • 9 of 11 males failed to sire any litters

♂ • however, female fertility is normal

reduced male fertility ( J:132072 )

♂ • 2 of 11 males each sired 1 small litter over a 4 month period when crossed to 6 different females

impaired fertilization ( J:132072 )

• following matings with superovulated females less than 0.5% of recovered eggs appear to be fertilized

• when sperm are incubated with cumulus free eggs only 3% of the eggs reach the 2-cell stage and none develop into blastocysts

• when sperm are incubated with zona pellucida-free eggs only 29% of the eggs reach the 2 cell stage and only 20% develop into blastocysts compared to about 87% (2-cell) and 69% (blastocyst) for heterozygous sperm

abnormal sperm physiology ( J:132072 )

♂ • lactate production by sperm in a 4 h period is about 10% that of controls

♂ • after 90 minutes or 4 hours of incubation in capacitating media ATP levels are about 52% or 82% lower, respectively, compared to wild-type controls

♂ • however, after 10 minutes of incubation ATP levels are similar to controls

asthenozoospermia ( J:132072 )

♂ • motility in freshly isolated sperm appears normal but after 4 h of incubation the percentage of motile sperm is reduced

♂ • after 1.5 h of incubation only 1.93% of sperm develop hyperactivity compared to 46.9% of heterozygous sperm

endocrine/exocrine glands

abnormal testis physiology ( J:132072 )

♂ • lactate dehydrogenase activity is reduced by about 50% compared to wild-type controls

cellular

asthenozoospermia ( J:132072 )

♂ • motility in freshly isolated sperm appears normal but after 4 h of incubation the percentage of motile sperm is reduced

♂ • after 1.5 h of incubation only 1.93% of sperm develop hyperactivity compared to 46.9% of heterozygous sperm