Phenotypes associated with this allele

• Allele Symbol: Ptprj^tm1.2Weis
• Allele Name: targeted mutation 1.2, Arthur Weiss
• Allele ID: MGI:3774766
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptprj^tm1.2Weis/Ptprj^tm1.2Weis	involves: 129P2/OlaHsd * BALB/cJ * C57BL/6	MGI:3774855
cx2	Ptprc^tm1Mak/Ptprc^tm1Mak Ptprj^tm1.2Weis/Ptprj^tm1.2Weis	B6.Cg-Ptprc^tm1Mak Ptprj^tm1.2Weis	MGI:3774857
cx3	Ptprc^tm1Mak/Ptprc^tm1Mak Ptprj^tm1.2Weis/Ptprj^tm1.2Weis	involves: 129/Sv * 129P2/OlaHsd * BALB/cJ * C57BL/6	MGI:3774856

Genotype
MGI:3774855

hm1

• Allelic Composition: Ptprj^tm1.2Weis/Ptprj^tm1.2Weis
• Genetic Background: involves: 129P2/OlaHsd * BALB/cJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal B cell differentiation ( J:131736 )

• partial block at T1 stage is observed

increased B-2 B cell number ( J:131736 )

• skewing toward B-2 lineage at expense of B-1 lineage is observed in peritoneal lavage B cells

increased marginal zone B cell number ( J:131736 )

• mice have more splenic marginal zone B cells than controls

abnormal splenic cell ratio ( J:131736 )

• spleens contain more T1 B cells (IgM^lo IgD^hi) and fewer follicular mature (FM, IgM^hi IgD^lo) B cells than controls

reproductive system

infertility ( J:131736 )

• homozygous mice are infertile; line is maintained as heterozygotes

immune system

abnormal B cell differentiation ( J:131736 )

• partial block at T1 stage is observed

increased B-2 B cell number ( J:131736 )

• skewing toward B-2 lineage at expense of B-1 lineage is observed in peritoneal lavage B cells

increased marginal zone B cell number ( J:131736 )

• mice have more splenic marginal zone B cells than controls

abnormal splenic cell ratio ( J:131736 )

• spleens contain more T1 B cells (IgM^lo IgD^hi) and fewer follicular mature (FM, IgM^hi IgD^lo) B cells than controls

Genotype
MGI:3774857

cx2

• Allelic Composition: Ptprc^tm1Mak/Ptprc^tm1Mak; Ptprj^tm1.2Weis/Ptprj^tm1.2Weis
• Genetic Background: B6.Cg-Ptprc^tm1Mak Ptprj^tm1.2Weis

mortality/aging

premature death ( J:131736 )

• mice die prematurely between 25 and 65 days of age

• Background Sensitivity: double mutants on mixed background (3 or less backcrosses to C57BL/6) show increased lifespan generally reaching 6 months of age and remaining healthy until 2 months of age, compared to accelerated phenotype of congenic mutants

growth/size/body

decreased body weight ( J:131736 )

• double null mice are significantly underweight (60% of weight of wild-type mice)

hematopoietic system

abnormal B cell differentiation ( J:131736 )

• pre B cell numbers are similar in double null and wild-type mice, but few B cells develop further to CD19+ CD43- B cells

decreased immature B cell number ( J:131736 )

• immature IgM+ IgD- B cells are decreased in number in the bone marrow

abnormal transitional stage B cell morphology ( J:131736 )

• there is a higher proportion of transitional stage B cells, but absolute B cell number is decreased

abnormal myelopoiesis ( J:131736 )

• spleens contain a cell population expressing CD19 but not IgM of IgD; cells are slightly bigger than normal B cells

• Background Sensitivity: myeloproliferative disorder with extramedullary hematopoiesis develops in congenic mice earlier than mice on mixed background

extramedullary hematopoiesis ( J:131736 )

• prior to death, mice develop a myeloproliferative disorder and show extramedullary hematopoiesis

anemia ( J:131736 )

• pre-terminal animals show varying degrees of anemia

decreased B cell number ( J:131736 )

• B cell lymphopenia develops; total cell numbers in spleens and lymph nodes are substatiantially lower than in wild-type mice or either single mutant homozygotes

• marked decreases in B cell numbers and percentages are observed in the bone marrow relative to controls

decreased mature B cell number ( J:131736 )

• practically no recirculating mature IgM+ IgD+ B cells are detected in the bone marrow

abnormal follicular B cell morphology ( J:131736 )

• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprj^tm1.2Weis cells

abnormal spleen morphology ( J:131736 )

• mice develop myeloproliferative disorder and show distortion of splenic architecture

immune system

abnormal B cell differentiation ( J:131736 )

• pre B cell numbers are similar in double null and wild-type mice, but few B cells develop further to CD19+ CD43- B cells

decreased immature B cell number ( J:131736 )

• immature IgM+ IgD- B cells are decreased in number in the bone marrow

abnormal transitional stage B cell morphology ( J:131736 )

• there is a higher proportion of transitional stage B cells, but absolute B cell number is decreased

abnormal myelopoiesis ( J:131736 )

• spleens contain a cell population expressing CD19 but not IgM of IgD; cells are slightly bigger than normal B cells

• Background Sensitivity: myeloproliferative disorder with extramedullary hematopoiesis develops in congenic mice earlier than mice on mixed background

decreased B cell number ( J:131736 )

• B cell lymphopenia develops; total cell numbers in spleens and lymph nodes are substatiantially lower than in wild-type mice or either single mutant homozygotes

• marked decreases in B cell numbers and percentages are observed in the bone marrow relative to controls

decreased mature B cell number ( J:131736 )

• practically no recirculating mature IgM+ IgD+ B cells are detected in the bone marrow

abnormal follicular B cell morphology ( J:131736 )

• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprj^tm1.2Weis cells

abnormal spleen morphology ( J:131736 )

• mice develop myeloproliferative disorder and show distortion of splenic architecture

liver inflammation ( J:131736 )

• extensive myeloid infiltration of liver is observed

lung inflammation ( J:131736 )

• scattered myeloid infiltrates are detected in the lungs

liver/biliary system

liver inflammation ( J:131736 )

• extensive myeloid infiltration of liver is observed

respiratory system

lung inflammation ( J:131736 )

• scattered myeloid infiltrates are detected in the lungs

Genotype
MGI:3774856

cx3

• Allelic Composition: Ptprc^tm1Mak/Ptprc^tm1Mak; Ptprj^tm1.2Weis/Ptprj^tm1.2Weis
• Genetic Background: involves: 129/Sv * 129P2/OlaHsd * BALB/cJ * C57BL/6

homeostasis/metabolism

abnormal B cell calcium ion homeostasis ( J:131736 )

• B cells show delayed calcium ion flux after B cell receptor crosslinking compared to wild-type and single null mice at 6-8 weeks of age

mortality/aging

premature death ( J:131736 )

• Background Sensitivity: on mixed background, mice live until 6 months and remain healthy until 2 months of age, compared to mice on congenic background which display earlier lethality and accelerated B cell phenotype

hematopoietic system

impaired macrophage phagocytosis ( J:131736 )

• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type

abnormal myelopoiesis ( J:131736 )

• Background Sensitivity: on mixed background, myeloproliferative disorder in mice is delayed in development compared to congenic mutants

decreased B cell number ( J:131736 )

• Background Sensitivity: young mice on mixed background show mild reduction in B cell number whereas reduction becomes more severe on congenic C57BL/6 background; B cell lymphopenia develops due to profound block at pre-B cell stage of development in the bone marrow

abnormal follicular B cell morphology ( J:131736 )

• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprj^tm1.2Weis cells

abnormal B cell calcium ion homeostasis ( J:131736 )

• B cells show delayed calcium ion flux after B cell receptor crosslinking compared to wild-type and single null mice at 6-8 weeks of age

immune system

impaired macrophage phagocytosis ( J:131736 )

• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type

abnormal myelopoiesis ( J:131736 )

• Background Sensitivity: on mixed background, myeloproliferative disorder in mice is delayed in development compared to congenic mutants

decreased B cell number ( J:131736 )

• Background Sensitivity: young mice on mixed background show mild reduction in B cell number whereas reduction becomes more severe on congenic C57BL/6 background; B cell lymphopenia develops due to profound block at pre-B cell stage of development in the bone marrow

abnormal follicular B cell morphology ( J:131736 )

• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprj^tm1.2Weis cells

abnormal B cell calcium ion homeostasis ( J:131736 )

• B cells show delayed calcium ion flux after B cell receptor crosslinking compared to wild-type and single null mice at 6-8 weeks of age

abnormal cytokine secretion ( J:131736 )

• Fc receptor-induced tumor necrosis factor alpha production (Tnfa) by bone marrow-derived macrophages in double mutant mice

• after LPS treatment Tnfa production by macrophages is equivalent to wild-type cells

cellular

impaired macrophage phagocytosis ( J:131736 )

• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type