Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptprjtm1.2Weis mutation
(2 available);
any
Ptprj mutation
(70 available)
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hematopoietic system
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• partial block at T1 stage is observed
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• skewing toward B-2 lineage at expense of B-1 lineage is observed in peritoneal lavage B cells
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• mice have more splenic marginal zone B cells than controls
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• spleens contain more T1 B cells (IgMlo IgDhi) and fewer follicular mature (FM, IgMhi IgDlo) B cells than controls
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reproductive system
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• homozygous mice are infertile; line is maintained as heterozygotes
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immune system
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• partial block at T1 stage is observed
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• skewing toward B-2 lineage at expense of B-1 lineage is observed in peritoneal lavage B cells
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• mice have more splenic marginal zone B cells than controls
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• spleens contain more T1 B cells (IgMlo IgDhi) and fewer follicular mature (FM, IgMhi IgDlo) B cells than controls
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mortality/aging
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• mice die prematurely between 25 and 65 days of age
• Background Sensitivity: double mutants on mixed background (3 or less backcrosses to C57BL/6) show increased lifespan generally reaching 6 months of age and remaining healthy until 2 months of age, compared to accelerated phenotype of congenic mutants
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growth/size/body
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• double null mice are significantly underweight (60% of weight of wild-type mice)
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hematopoietic system
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• pre B cell numbers are similar in double null and wild-type mice, but few B cells develop further to CD19+ CD43- B cells
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• immature IgM+ IgD- B cells are decreased in number in the bone marrow
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• there is a higher proportion of transitional stage B cells, but absolute B cell number is decreased
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• spleens contain a cell population expressing CD19 but not IgM of IgD; cells are slightly bigger than normal B cells
• Background Sensitivity: myeloproliferative disorder with extramedullary hematopoiesis develops in congenic mice earlier than mice on mixed background
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• prior to death, mice develop a myeloproliferative disorder and show extramedullary hematopoiesis
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• pre-terminal animals show varying degrees of anemia
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• B cell lymphopenia develops; total cell numbers in spleens and lymph nodes are substatiantially lower than in wild-type mice or either single mutant homozygotes
• marked decreases in B cell numbers and percentages are observed in the bone marrow relative to controls
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• practically no recirculating mature IgM+ IgD+ B cells are detected in the bone marrow
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• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprjtm1.2Weis cells
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• mice develop myeloproliferative disorder and show distortion of splenic architecture
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immune system
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• pre B cell numbers are similar in double null and wild-type mice, but few B cells develop further to CD19+ CD43- B cells
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• immature IgM+ IgD- B cells are decreased in number in the bone marrow
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• there is a higher proportion of transitional stage B cells, but absolute B cell number is decreased
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• spleens contain a cell population expressing CD19 but not IgM of IgD; cells are slightly bigger than normal B cells
• Background Sensitivity: myeloproliferative disorder with extramedullary hematopoiesis develops in congenic mice earlier than mice on mixed background
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• B cell lymphopenia develops; total cell numbers in spleens and lymph nodes are substatiantially lower than in wild-type mice or either single mutant homozygotes
• marked decreases in B cell numbers and percentages are observed in the bone marrow relative to controls
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• practically no recirculating mature IgM+ IgD+ B cells are detected in the bone marrow
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• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprjtm1.2Weis cells
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• mice develop myeloproliferative disorder and show distortion of splenic architecture
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• extensive myeloid infiltration of liver is observed
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• scattered myeloid infiltrates are detected in the lungs
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liver/biliary system
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• extensive myeloid infiltration of liver is observed
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respiratory system
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• scattered myeloid infiltrates are detected in the lungs
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mortality/aging
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• Background Sensitivity: on mixed background, mice live until 6 months and remain healthy until 2 months of age, compared to mice on congenic background which display earlier lethality and accelerated B cell phenotype
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hematopoietic system
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• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type
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• Background Sensitivity: on mixed background, myeloproliferative disorder in mice is delayed in development compared to congenic mutants
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• Background Sensitivity: young mice on mixed background show mild reduction in B cell number whereas reduction becomes more severe on congenic C57BL/6 background; B cell lymphopenia develops due to profound block at pre-B cell stage of development in the bone marrow
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• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprjtm1.2Weis cells
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• B cells show delayed calcium ion flux after B cell receptor crosslinking compared to wild-type and single null mice at 6-8 weeks of age
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immune system
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• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type
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• Background Sensitivity: on mixed background, myeloproliferative disorder in mice is delayed in development compared to congenic mutants
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• Background Sensitivity: young mice on mixed background show mild reduction in B cell number whereas reduction becomes more severe on congenic C57BL/6 background; B cell lymphopenia develops due to profound block at pre-B cell stage of development in the bone marrow
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• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprjtm1.2Weis cells
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• B cells show delayed calcium ion flux after B cell receptor crosslinking compared to wild-type and single null mice at 6-8 weeks of age
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• Fc receptor-induced tumor necrosis factor alpha production (Tnfa) by bone marrow-derived macrophages in double mutant mice
• after LPS treatment Tnfa production by macrophages is equivalent to wild-type cells
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cellular
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• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type
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Analysis Tools