All Phenotypes Notch1<tm2Pst> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Notch1^tm2Pst/Notch1^tm2Pst	B6.Cg-Notch1^tm2Pst	MGI:3775286
ht2	Notch1^tm2Pst/Notch1^tm2.1Pst	B6.Cg-Notch1^tm2.1Pst	MGI:3775287
ht3	Notch1^tm2Pst/Notch1^tm2.1Pst	involves: 129/Sv * C57BL/6 * SJL	MGI:3775288

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased T cell apoptosis ( J:131563 )

• thymocytes, more specifically double positive T cells, exhibit increased levels of apoptosis compared to wild-type thymocytes

abnormal T cell differentiation ( J:131563 )

• all of the more mature T cell subsets are decreased compared to in wild-type mice

• mice exhibit fewer CD45.2+ double positive T cells following donor transplant than wild-type mice

• the transition from double positive to single positive is enhanced

• however, emigration of T cells to the spleen is normal

decreased double-negative T cell number ( J:131563 )

• the number of CD44-CD25- DN4 cells is decreased 40% compared to in wild-type mice

• however, the number of DN3 T cells is slightly increased

decreased T cell number ( J:131563 )

• all of the more mature T cell subsets are decreased compared to in wild-type mice

decreased thymocyte number ( J:131563 )

• the thymus contains 50% fewer thymocytes than in wild-type mice

• however, emigration of T cells to the spleen is normal

growth/size/body

postnatal growth retardation ( J:131563 )

• mice grow slower than wild-type mice after weaning

hematopoietic system

increased T cell apoptosis ( J:131563 )

• thymocytes, more specifically double positive T cells, exhibit increased levels of apoptosis compared to wild-type thymocytes

abnormal T cell differentiation ( J:131563 )

• all of the more mature T cell subsets are decreased compared to in wild-type mice

• mice exhibit fewer CD45.2+ double positive T cells following donor transplant than wild-type mice

• the transition from double positive to single positive is enhanced

• however, emigration of T cells to the spleen is normal

decreased T cell number ( J:131563 )

• all of the more mature T cell subsets are decreased compared to in wild-type mice

decreased thymocyte number ( J:131563 )

• the thymus contains 50% fewer thymocytes than in wild-type mice

• however, emigration of T cells to the spleen is normal

decreased double-negative T cell number ( J:131563 )

• the number of CD44-CD25- DN4 cells is decreased 40% compared to in wild-type mice

• however, the number of DN3 T cells is slightly increased

cellular

increased T cell apoptosis ( J:131563 )

• thymocytes, more specifically double positive T cells, exhibit increased levels of apoptosis compared to wild-type thymocytes

endocrine/exocrine glands

decreased thymocyte number ( J:131563 )

• the thymus contains 50% fewer thymocytes than in wild-type mice

• however, emigration of T cells to the spleen is normal

Allelic Composition	Notch1^tm2Pst/Notch1^tm2.1Pst
Genetic Background	B6.Cg-Notch1^tm2.1Pst

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1^tm2.1Pst mutation (0 available); any Notch1 mutation (115 available)
Notch1^tm2Pst mutation (0 available); any Notch1 mutation (115 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:131563 )

• mice die at E10

embryo

embryonic growth retardation ( J:131563 )

• at E9.5, mice exhibit 13 to 17 somites compared to 23 to 26 in Notch1^tm2Pst homozygotes

growth/size/body

embryonic growth retardation ( J:131563 )

• at E9.5, mice exhibit 13 to 17 somites compared to 23 to 26 in Notch1^tm2Pst homozygotes

Allelic Composition	Notch1^tm2Pst/Notch1^tm2.1Pst
Genetic Background	involves: 129/Sv * C57BL/6 * SJL

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:131563 )

• mice die around E12

embryo

embryonic growth retardation ( J:131563 )

• at E9.5, mice exhibit 17 to 21 somites compared to 23 to 26 in Notch1^tm2Pst homozygotes and 13 to 17 in Notch1^tm2.1Pst homozygotes

decreased embryo size ( J:131563 )

• at E10.5

abnormal embryonic neuroepithelium morphology ( J:131563 )

• at E10.5

cardiovascular system

abnormal vasculogenesis ( J:131563 )

• at E10.5 mice are anemic due to defects in vasculogenesis

abnormal heart development ( J:131563 )

• at E10.5, mice exhibit an underdeveloped heart loop

nervous system

abnormal embryonic neuroepithelium morphology ( J:131563 )

• at E10.5

hematopoietic system

anemia ( J:131563 )

• at E10.5 mice are anemic due to defects in vasculogenesis

growth/size/body

embryonic growth retardation ( J:131563 )

• at E9.5, mice exhibit 17 to 21 somites compared to 23 to 26 in Notch1^tm2Pst homozygotes and 13 to 17 in Notch1^tm2.1Pst homozygotes

decreased embryo size ( J:131563 )

• at E10.5

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/30/2024 MGI 6.23