Phenotypes associated with this allele

• Allele Symbol: Ighm^tm3Bgmn
• Allele Name: targeted mutation 3, Marianne Bruggemann
• Allele ID: MGI:3774509
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ighm^tm3Bgmn/Ighm^tm3Bgmn	involves: 129	MGI:3806673
cx2	Ighm^tm3Bgmn/Ighm^tm3Bgmn Igkc^tm1Bgmn/Igkc^tm1Bgmn Igl^tm2.1Bgmn/Igl^tm2.1Bgmn	involves: 129P2/OlaHsd * 129/Sv	MGI:3806698

Genotype
MGI:3806673

hm1

• Allelic Composition: Ighm^tm3Bgmn/Ighm^tm3Bgmn
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased immature B cell number ( J:131633 )

• immature B cells in the bone marrow are reduced by 40% to 50%

decreased mature B cell number ( J:131633 )

• B220⁺ IgM⁺ IgD⁺ B cells in the spleen are reduced by 40%

• immunized mice have half the number of B cells in the spleen as immunized wild-type mice

increased B-1 B cell number ( J:131633 )

• CD5⁺ B-1 B cells are greatly increased in the peritoneum

• CD5⁺ B-1 B cells outnumber CD5^- B1-B cells in the peritoneum while the opposite occurs in wild-type mice

• there is about a 3-fold increase of B1-B cells in the spleen and bone marrow

abnormal B cell activation ( J:131633 )

• B cells have poor and delayed calcium mobilization in response to activation

decreased B cell proliferation ( J:131633 )

• LPS induced B cell proliferation is decreased by 50% compared to controls

• BCR-mediated proliferation is 5-fold less than controls

decreased IgM level ( J:131633 )

• some IgM is detectable in the sera of unimmunized mice but these IgM molecules consist of monomer subunits and not the normal pentameric structure

• immunization with antigens leads to a small increase in sera IgM monomers but only to levels 10% that of controls

• these monomer IgM antibodies lack specificity to the antigen used in immunization

hematopoietic system

decreased immature B cell number ( J:131633 )

• immature B cells in the bone marrow are reduced by 40% to 50%

decreased mature B cell number ( J:131633 )

• B220⁺ IgM⁺ IgD⁺ B cells in the spleen are reduced by 40%

• immunized mice have half the number of B cells in the spleen as immunized wild-type mice

increased B-1 B cell number ( J:131633 )

• CD5⁺ B-1 B cells are greatly increased in the peritoneum

• CD5⁺ B-1 B cells outnumber CD5^- B1-B cells in the peritoneum while the opposite occurs in wild-type mice

• there is about a 3-fold increase of B1-B cells in the spleen and bone marrow

abnormal B cell activation ( J:131633 )

• B cells have poor and delayed calcium mobilization in response to activation

decreased B cell proliferation ( J:131633 )

• LPS induced B cell proliferation is decreased by 50% compared to controls

• BCR-mediated proliferation is 5-fold less than controls

decreased IgM level ( J:131633 )

• some IgM is detectable in the sera of unimmunized mice but these IgM molecules consist of monomer subunits and not the normal pentameric structure

• immunization with antigens leads to a small increase in sera IgM monomers but only to levels 10% that of controls

• these monomer IgM antibodies lack specificity to the antigen used in immunization

cellular

decreased B cell proliferation ( J:131633 )

• LPS induced B cell proliferation is decreased by 50% compared to controls

• BCR-mediated proliferation is 5-fold less than controls

Genotype
MGI:3806698

cx2

• Allelic Composition: Ighm^tm3Bgmn/Ighm^tm3Bgmn; Igkc^tm1Bgmn/Igkc^tm1Bgmn; Igl^tm2.1Bgmn/Igl^tm2.1Bgmn
• Genetic Background: involves: 129P2/OlaHsd * 129/Sv

hematopoietic system

abnormal B cell differentiation ( J:130864 )

• B cell development is partially blocked at the small pre-B cell stage but the expression of a heavy chain-only BCR overcomes this block

• there is a B cell population that makes up 8.4% of splenocytes that express Igbeta

decreased IgG level ( J:130864 )

• reduced but detectable amounts of IgG antibody are found in the sera

• antibodies consist of heavy chain only covalently linked homodimers

decreased IgM level ( J:130864 )

• IgM is present at reduced amounts in the sera

• IgM consist only of heavy chains that are non-covalently linked

immune system

abnormal B cell differentiation ( J:130864 )

• B cell development is partially blocked at the small pre-B cell stage but the expression of a heavy chain-only BCR overcomes this block

• there is a B cell population that makes up 8.4% of splenocytes that express Igbeta

decreased IgG level ( J:130864 )

• reduced but detectable amounts of IgG antibody are found in the sera

• antibodies consist of heavy chain only covalently linked homodimers

decreased IgM level ( J:130864 )

• IgM is present at reduced amounts in the sera

• IgM consist only of heavy chains that are non-covalently linked