All Phenotypes Stat1<tm1Tdec> MGI Mouse

abnormal macrophage physiology ( J:130622 )

• the anti-bacterial activity of macrophages is impaired in these mice

• bone-marrow derived macrophages pre-treated with IFN-gamma have higher intracellular titers of Listeria monocytogenes 3 and 6 hours after in vitro infection

• titers are lower than those found in macrophages from Stat1^tm1Dlv homozygotes

abnormal MHC II cell surface expression on macrophages ( J:130622 )

• there are no high expressers of class II among a population of bone marrow derived macrophages

decreased susceptibility to endotoxin shock ( J:130622 )

• only 9% of mice die within 70 hours of 25 mg/kg body weight of LPS administration while 46% of wild-type mice die within 46 hours

• 80% of mice die at a dose of 37 mg/kg body weight of LPS but with a significant delay compared to wild-type mice

increased susceptibility to bacterial infection ( J:130622 )

• only 80% of the mice survive when infected with 10⁵ Listeria monocytogenes bacteria while all wild-type mice survive

• all mice die when infected with 10⁶ L. monocytogenes bacteria while 80% of wild-type mice survive

• clearance of L. monocytogenes bacteria from the spleen and liver is reduced by 10-fold compared to wild-type mice 3 days after infection

increased susceptibility to Riboviria infection ( J:130622 )

• primary fibroblasts need to be pre-treated with higher doses of IFN-gamma than wild-type fibroblasts do to survive exposure to vesicular stomatitis for 24 hours

• this partial protection is better than observed in fibroblasts from Stat1^tm1Dlv homozygotes