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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		E2f7tm1.1Gle
targeted mutation 1.1, Gustavo Leone
MGI:3773722
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		E2f7tm1.1Gle/E2f7tm1.1Gle involves: 129S6/SvEvTac * FVB/N MGI:3797852
cx2
 		E2f7tm1.1Gle/E2f7tm1.1Gle
E2f8tm1.1Gle/E2f8tm1.1Gle 		involves: 129S6/SvEvTac MGI:5473264
cx3
 		E2f3tm2.1Gle/E2f3tm2.1Gle
E2f7tm1.1Gle/E2f7tm1.1Gle
E2f8tm1.1Gle/E2f8tm1.1Gle 		involves: 129S6/SvEvTac MGI:5473265
cx4
 		E2f7tm1.1Gle/E2f7tm1.1Gle
E2f8tm1.1Gle/E2f8tm1.1Gle 		involves: 129S6/SvEvTac * FVB/N MGI:3797854
cx5
 		E2f7tm1.1Gle/E2f7tm1.1Gle
E2f8tm1.1Gle/E2f8+ 		involves: 129S6/SvEvTac * FVB/N MGI:3797862
cx6
 		E2f7tm1.1Gle/E2f7+
E2f8tm1.1Gle/E2f8tm1.1Gle 		involves: 129S6/SvEvTac * FVB/N MGI:3797864


Genotype
MGI:3797852
hm1
Allelic
Composition		 E2f7tm1.1Gle/E2f7tm1.1Gle
Genetic
Background		 involves: 129S6/SvEvTac * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f7tm1.1Gle mutation (0 available); any E2f7 mutation (32 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:131292 )
• pups develop normally through puberty and live to old age




Genotype
MGI:5473264
cx2
Allelic
Composition		 E2f7tm1.1Gle/E2f7tm1.1Gle
E2f8tm1.1Gle/E2f8tm1.1Gle
Genetic
Background		 involves: 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f7tm1.1Gle mutation (0 available); any E2f7 mutation (32 available)
E2f8tm1.1Gle mutation (0 available); any E2f8 mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:183999 )
• no live embryos are recovered after E10.5
• double mutants with wild-type (Sox2-cre E2f7fl/- E28fl/-) placentas appear normal at E10.5 and lack the developmental phenotypes in double null embryos
• Note: Sox2-cre is expressed only in the inner cell mass with no extraembronic tissue expression so the placentas are normal
• however, embryos rescued from lethality during development die within a few days of birth

growth/size/body
fetal growth retardation ( J:183999 )

embryo
abnormal placental labyrinth vasculature morphology ( J:183999 )
• vascular network is poorly formed, with maternal sinusoids rarely adjacent to fetal blood vessels
increased placenta apoptosis ( J:183999 )
• placentas have an increased number of apoptotic spongiotrophoblast cells
abnormal embryo development ( J:183999 )
• embryos show profound stress response
• widespread apoptosis is observed in embryos
small placenta ( J:183999 )
• placentas are smaller overall
abnormal trophoblast layer morphology ( J:183999 )
• abnormally large clusters of densely packed trophoblast cells which do not effectively invade the maternal decidua
abnormal spongiotrophoblast cell morphology ( J:183999 )
• placentas have an increased number of apoptotic spongiotrophoblast cells
abnormal placenta development ( J:183999 )
• placentas display altered differentiation of multiple extraembryonic cell lineages

cellular
increased placenta apoptosis ( J:183999 )
• placentas have an increased number of apoptotic spongiotrophoblast cells

cardiovascular system
abnormal placental labyrinth vasculature morphology ( J:183999 )
• vascular network is poorly formed, with maternal sinusoids rarely adjacent to fetal blood vessels




Genotype
MGI:5473265
cx3
Allelic
Composition		 E2f3tm2.1Gle/E2f3tm2.1Gle
E2f7tm1.1Gle/E2f7tm1.1Gle
E2f8tm1.1Gle/E2f8tm1.1Gle
Genetic
Background		 involves: 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f3tm2.1Gle mutation (0 available); any E2f3 mutation (32 available)
E2f7tm1.1Gle mutation (0 available); any E2f7 mutation (32 available)
E2f8tm1.1Gle mutation (0 available); any E2f8 mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
embryo
embryo phenotype ( J:183999 )
N
• placentas appear normal with well-organized labyrinthine architecture and vasculature, and are functional as fetuses lack signs of acute stress response or ectopic cell apoptosis
• live embryos/fetuses are recovered at all stages analyzed, including at birth




Genotype
MGI:3797854
cx4
Allelic
Composition		 E2f7tm1.1Gle/E2f7tm1.1Gle
E2f8tm1.1Gle/E2f8tm1.1Gle
Genetic
Background		 involves: 129S6/SvEvTac * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f7tm1.1Gle mutation (0 available); any E2f7 mutation (32 available)
E2f8tm1.1Gle mutation (0 available); any E2f8 mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:131292 )
• by E10.5, only 45% of double mutants are alive and by E11.5 all are dead

growth/size/body
decreased embryo size ( J:131292 )
• smaller at E9.5

embryo
abnormal vitelline vasculature morphology ( J:131292 )
• live E10.5 embryos often have vascular defects in the yolk sac
increased embryonic tissue cell apoptosis ( J:131292 )
• massive apoptosis in embryos at E9.5; multiple areas within the head mesenchyme, branchial arch, somites, and neural tube contain cells with pyknotic nuclei surrounded by bright eosinophilic cytoplasm
decreased embryo size ( J:131292 )
• smaller at E9.5

cardiovascular system
abnormal blood vessel morphology ( J:131292 )
• large dilated blood vessels
abnormal vitelline vasculature morphology ( J:131292 )
• live E10.5 embryos often have vascular defects in the yolk sac
hemorrhage ( J:131292 )
• large dilated blood vessels associated with multifocal hemorrhages

cellular
increased embryonic tissue cell apoptosis ( J:131292 )
• massive apoptosis in embryos at E9.5; multiple areas within the head mesenchyme, branchial arch, somites, and neural tube contain cells with pyknotic nuclei surrounded by bright eosinophilic cytoplasm




Genotype
MGI:3797862
cx5
Allelic
Composition		 E2f7tm1.1Gle/E2f7tm1.1Gle
E2f8tm1.1Gle/E2f8+
Genetic
Background		 involves: 129S6/SvEvTac * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f7tm1.1Gle mutation (0 available); any E2f7 mutation (32 available)
E2f8tm1.1Gle mutation (0 available); any E2f8 mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:131292 )
• most die within the first 3 months of life

growth/size/body
decreased body size ( J:131292 )
• most mutants appear runted




Genotype
MGI:3797864
cx6
Allelic
Composition		 E2f7tm1.1Gle/E2f7+
E2f8tm1.1Gle/E2f8tm1.1Gle
Genetic
Background		 involves: 129S6/SvEvTac * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f7tm1.1Gle mutation (0 available); any E2f7 mutation (32 available)
E2f8tm1.1Gle mutation (0 available); any E2f8 mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:131292 )
• mutants appear developmentally normal




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory