Analysis Tools
mortality/aging
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• mice are viable and indistinguishable in general appearance from wild-type
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reproductive system
 N |
• mutant testes are of normal size, comparable morphology and sperm density with germ cells found in seminiferous tubules at all stages of testes development as well as Leydig and Sertoli cells; epididymides have similar morphology to controls
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• central pair apparatus is intact, but periaxonemal structure is abnormal
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• the fibrous sheath normally surrounding the axoneme is punctured and distorted with visible gaps in the principal piece
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• the fibrous sheath normally surrounding the axoneme is punctured and distorted
• 8 + 3 positioning of longitudinal columns in flagella is present, but in more than 50% of principal piece analyzed, 1 or 2 extra columns are found at random positions; such additional columns are accompanied by emergence of a longitudinal column anchor between the extra one and its corresponding doublet
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• sperm count in the testes is slightly reduced by about 14%; in the cauda epididymis, sperm count is reduced by about 30%
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• almost all remaining sperm heads have a club-shaped morphology
• sperm heads display a large gap between the acrosome and the nucleus
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• nucleus shape is altered in spermatids (elongated and narrowed)
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• more than 36% of sperm are decapitated
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• manchette develops pincer-like appearance
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• in elongating spermatids, the manchette can be excessively elongated
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• over 50% of capacitated spermatozoa show no mobility; about 23% display progressive movement while 26% are mobile, showing bending of the flagellum but inability to propagate the waveform along the flagellum
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• over 50% of capacitated spermatozoa show no mobility; about 23% display progressive movement while 26% are mobile, showing bending of the flagellum but inability to propagate the waveform along the flagellum
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• when tested for fertility, it is observed that although vaginal plugs are found in females no offspring are produced from the matings
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cellular
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• central pair apparatus is intact, but periaxonemal structure is abnormal
|
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• the fibrous sheath normally surrounding the axoneme is punctured and distorted with visible gaps in the principal piece
|
|
|
• the fibrous sheath normally surrounding the axoneme is punctured and distorted
• 8 + 3 positioning of longitudinal columns in flagella is present, but in more than 50% of principal piece analyzed, 1 or 2 extra columns are found at random positions; such additional columns are accompanied by emergence of a longitudinal column anchor between the extra one and its corresponding doublet
|
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• sperm count in the testes is slightly reduced by about 14%; in the cauda epididymis, sperm count is reduced by about 30%
|
|
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• almost all remaining sperm heads have a club-shaped morphology
• sperm heads display a large gap between the acrosome and the nucleus
|
|
|
• nucleus shape is altered in spermatids (elongated and narrowed)
|
|
|
• more than 36% of sperm are decapitated
|
|
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• manchette develops pincer-like appearance
|
|
|
• in elongating spermatids, the manchette can be excessively elongated
|
|
|
• over 50% of capacitated spermatozoa show no mobility; about 23% display progressive movement while 26% are mobile, showing bending of the flagellum but inability to propagate the waveform along the flagellum
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• over 50% of capacitated spermatozoa show no mobility; about 23% display progressive movement while 26% are mobile, showing bending of the flagellum but inability to propagate the waveform along the flagellum
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