Phenotypes associated with this allele
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plcg2tm1.1Kuro mutation
(0 available);
any
Plcg2 mutation
(74 available)
|
|
|
immune system
|
|
• unlike wild-type cells, cytokine-activated natural killer cells fail to kill YAC-1 or BaF/3-Rae1gamma cells
|
|
|
• unlike wild-type cells, NKG2D-stimulated natural killer (NK) cells fail to produce IFN-gamma
• however, NK cells produce large amounts of IFN-gamma when exposed to YAC-1 cells
|
hematopoietic system
|
|
• unlike wild-type cells, cytokine-activated natural killer cells fail to kill YAC-1 or BaF/3-Rae1gamma cells
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plcg2tm1.1Kuro mutation
(0 available);
any
Plcg2 mutation
(74 available)
|
|
|
immune system
|
|
• spleen cellularity is increased by about 50% compared to controls
|
|
|
• B cell development is partially blocked at an early transition stage but not to the degree that Blnk null homozygotes are
• evidence includes a lower number of peripheral B cells, an increase percentage of B220+ IgL- cells and a 10-fold increase in the number of marrow cells expressing pre-BCR
|
|
|
• there is an increase in B220+ IgL- cells in the bone marrow
|
|
|
• NK cells exhibit partially reduced conjugation with RMA-S cells compared with wild-type cells
• decreased killing of hematopoietic cells RMA-S and YAC-1 cells
|
hematopoietic system
|
|
• spleen cellularity is increased by about 50% compared to controls
|
|
|
• B cell development is partially blocked at an early transition stage but not to the degree that Blnk null homozygotes are
• evidence includes a lower number of peripheral B cells, an increase percentage of B220+ IgL- cells and a 10-fold increase in the number of marrow cells expressing pre-BCR
|
|
|
• there is an increase in B220+ IgL- cells in the bone marrow
|
|
|
• NK cells exhibit partially reduced conjugation with RMA-S cells compared with wild-type cells
• decreased killing of hematopoietic cells RMA-S and YAC-1 cells
|
growth/size/body
|
|
• spleen cellularity is increased by about 50% compared to controls
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Blnktm1Kplm mutation
(0 available);
any
Blnk mutation
(76 available)
Plcg2tm1.1Kuro mutation
(0 available);
any
Plcg2 mutation
(74 available)
|
|
|
mortality/aging
|
|
• many mice have perinatal deaths due to a bleeding disorder associated with PLCgamma-2 deficiency
• at weaning, litters from double heterozygote parents only yielded 1 out of 40 pups that were double homozygotes
|
immune system
|
|
• 40% of B cells are dead after 2 hours in culture with 60% after 4 hours and 80% dead after six hours
|
|
|
• B cell lineage cells are reduced 3- to 5-fold compared to mice null homozygotes for just one of the alleles
• splenic B cell numbers are reduced to 4.1% of lymphocytes
|
|
|
• spleen cellularity is about one half of that in wild-type mice
|
hematopoietic system
|
|
• 40% of B cells are dead after 2 hours in culture with 60% after 4 hours and 80% dead after six hours
|
|
|
• B cell lineage cells are reduced 3- to 5-fold compared to mice null homozygotes for just one of the alleles
• splenic B cell numbers are reduced to 4.1% of lymphocytes
|
|
|
• spleen cellularity is about one half of that in wild-type mice
|
cellular
|
|
• 40% of B cells are dead after 2 hours in culture with 60% after 4 hours and 80% dead after six hours
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Blnktm1Kplm mutation
(0 available);
any
Blnk mutation
(76 available)
Plcg2tm1.1Kuro mutation
(0 available);
any
Plcg2 mutation
(74 available)
|
|
|
immune system
|
|
• 40% of B cells are dead after 2 hours in culture with 50% after 4 hours and 70% dead after 6 hours
|
|
|
• splenic B cell numbers are reduced to 13.5% of lymphocytes
|
|
|
• there is a 4.3 fold decrease in the concentration of calcium fluxed intracellularly after stimulus compared to wild-type controls
|
hematopoietic system
|
|
• 40% of B cells are dead after 2 hours in culture with 50% after 4 hours and 70% dead after 6 hours
|
|
|
• splenic B cell numbers are reduced to 13.5% of lymphocytes
|
|
|
• there is a 4.3 fold decrease in the concentration of calcium fluxed intracellularly after stimulus compared to wild-type controls
|
cellular
|
|
• 40% of B cells are dead after 2 hours in culture with 50% after 4 hours and 70% dead after 6 hours
|
Analysis Tools