Phenotypes associated with this allele

• Allele Symbol: Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri
• Allele Name: transgene insertion 187-7, Ralph L Brinster
• Allele ID: MGI:3766019
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Ins2-CD86)12B70Flv/0 Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri/0	involves: C57BL/6 * CBA/Ca * SJL	MGI:3766040
cx2	Tg(Ins2-CD80)3B7Flv/0 Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri/0	involves: C57BL/6 * CBA/Ca * SJL	MGI:3766042
tg3	Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri/0	involves: C57BL/6 * SJL	MGI:3766041

Genotype
MGI:3766040

cx1

• Allelic Composition: Tg(Ins2-CD86)12B70Flv/0; Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri/0
• Genetic Background: involves: C57BL/6 * CBA/Ca * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:93555 )

• diabetes-like symptoms (dehydration, weight loss) lead to death of most animals by 6 months of age

growth/size/body

weight loss ( J:93555 )

immune system

insulitis ( J:93555 )

• double mutants develop insulitis at 2 months of age

abnormal cytokine secretion ( J:93555 )

• mice have a greater number of cytokine-positive cells (Il4 or Ifng) compared to mice coexpressing CD80 and the MHC class II transgene

increased susceptibility to autoimmune diabetes ( J:93555 )

• mice develop clinical signs of diabetes

endocrine/exocrine glands

decreased pancreatic beta cell number ( J:93555 )

• mice show autoimmune destruction of pancreatic beta cells

insulitis ( J:93555 )

• double mutants develop insulitis at 2 months of age

homeostasis/metabolism

increased circulating glucose level ( J:93555 )

• mice show elevated blood glucose levels (363 mg/dl at 8 weeks rising to 402 mg/dl at 6 months)

dehydration ( J:93555 )

Genotype
MGI:3766042

cx2

• Allelic Composition: Tg(Ins2-CD80)3B7Flv/0; Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri/0
• Genetic Background: involves: C57BL/6 * CBA/Ca * SJL

mortality/aging

premature death ( J:93555 )

• diabetes-like symptoms (dehydration, weight loss) lead to death of most animals by 6 months of age

growth/size/body

weight loss ( J:93555 )

immune system

insulitis ( J:93555 )

• mice develop insulitis starting at ~5 months of age

increased susceptibility to autoimmune diabetes ( J:93555 )

• mice develop clinical signs of diabetes

endocrine/exocrine glands

abnormal pancreatic islet morphology ( J:93555 )

• islet architecture becomes disorganized and islet tissue is replace by a ductal hyperplasia

insulitis ( J:93555 )

• mice develop insulitis starting at ~5 months of age

homeostasis/metabolism

dehydration ( J:93555 )

Genotype
MGI:3766041

tg3

• Allelic Composition: Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri/0
• Genetic Background: involves: C57BL/6 * SJL

immune system

decreased susceptibility to autoimmune diabetes ( J:93555 )

• transgenic mice do not develop autoimmune diabetes

homeostasis/metabolism

decreased insulin secretion ( J:127706 )

• islets from transgenic diabetic animals stain weakly for insulin compared to controls

increased circulating glucose level ( J:93555 , J:127706 )

• blood glucose levels are ~300 mg.dl at 8 weeks, increasing to 412 mg/dl by 6 months (J:93555)

• transgenic animals show elevated blood glucose throughout life (J:127706)

hyperglycemia ( J:127706 )

• diabetes, developed by all transgenic animals, is characterized by onset of hyperglycemia at 3-5 weeks of age

• blood glucose level at 6-10 weeks is 160-1055 mg/dl increasing to 543-1255 mg/dl at 10 weeks compared to 124-223 mg/dl in controls

• levels are >500 mg/dl in most animals by 2 months of age

increased circulating triglyceride level ( J:127706 )

• serum is grossly lipemic in transgenic mice; some diabetic animals have levels above 120 mg/dl

ketoaciduria ( J:127706 )

• urine tests positive for presence of ketones; ketonuria correlates with severity of diabetes

endocrine/exocrine glands

decreased insulin secretion ( J:127706 )

• islets from transgenic diabetic animals stain weakly for insulin compared to controls

renal/urinary system

ketoaciduria ( J:127706 )

• urine tests positive for presence of ketones; ketonuria correlates with severity of diabetes

polyuria ( J:127706 )

• accompanies rise in blood glucose and polydispsia

behavior/neurological

polydipsia ( J:127706 )

• at 8 weeks, water consumption is observed to be ~50 ml/day compared to ~4.2 ml/day in controls