Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lats2tm1Noj mutation
(1 available);
any
Lats2 mutation
(42 available)
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cellular
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• mouse embryonic fibroblasts exhibit centrosomal fragmentation that is reduced over long-term passage unlike wild-type cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lats2tm1Noj mutation
(1 available);
any
Lats2 mutation
(42 available)
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Lats2tm1Noj/Lats2tm1Noj embryos exhibit severe nervous system developmental defects
mortality/aging
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• while 30 embryos are recovered at E10.5, only 3 embryos are recovered at E11.5 and none are recovered at E12.5 or later
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embryo
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• at E13.5, branchial arches exhibit delayed development
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• at E13.5, growth retardation is evident in the whole embryo and in a number of organs including branchial arches and skeletal muscle
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cellular
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• 24% of mouse embryonic fibroblasts contain an increase in centrosome number (greater than 2 per cell) where 7.6% of wild-type cells have greater than 2 centrosomes
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• 15.1% of mouse embryonic fibroblasts have an enlarged nucleus compared to only 1.7% of wild-type cells
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• 3.4-fold more mouse fibroblast cells are multinucleated relative to wild-type cells
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• some mouse embryonic fibroblast cells show polyploidy
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• the number mouse embryonic fibroblast cells that fail cytokinesis is increased 3.3-fold relative to wild-type cells
• mouse embryonic fibroblast cells exit mitosis faster than wild-type cells
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• at E13.5, apoptotic cells are observed in the spinal cord
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nervous system
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• at E13.5, apoptotic cells are observed in the spinal cord
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• at E13.5, surviving mice have a reduced number of nerve cells in the neuroepithelial layer of the fourth ventricle as well as infiltration of inflammatory cells
• at E13.5, the neuroepithelial layer is thinner and contains many pyknotic cells
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• at E10.5 and E13.5, spinal cords have reduced number of nerve cells and the density of nerve fibers
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muscle
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• at E13.5, skeletal muscle development is delayed
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growth/size/body
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• at E13.5, growth retardation is evident in the whole embryo and in a number of organs including branchial arches and skeletal muscle
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cardiovascular system
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• at E13.5, surviving mice have small hearts with only modest developmental retardation
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craniofacial
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• at E13.5, branchial arches exhibit delayed development
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Allelic
Composition |
Lats2tm1Noj/Lats2+
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Genetic
Background |
involves: 129S2/SvPas * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lats2tm1Noj mutation
(1 available);
any
Lats2 mutation
(42 available)
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mortality/aging
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• males embryos experience more embryonic lethality than females
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Analysis Tools
