Phenotypes associated with this allele

• Allele Symbol: Tg(tetO-KLF4)32831Rup
• Allele Name: transgene insertion 32831, J Michael Ruppert
• Allele ID: MGI:3717763
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Trp53^tm1Brd/Trp53^tm1Brd Tg(KRT14-rtTA)208Jek/Y Tg(tetO-KLF4)32831Rup/0	involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6NTac * SJL	MGI:3717875
cx2	Trp53^tm1Brd/Trp53^+ Tg(KRT14-rtTA)208Jek/0 Tg(tetO-KLF4)32831Rup/0	involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6NTac * SJL	MGI:3717876
cx3	Tg(KRT14-rtTA)208Jek/Y Tg(tetO-KLF4)32831Rup/0	involves: C57BL/6 * SJL	MGI:3717902

Genotype
MGI:3717875

cx1

• Allelic Composition: Trp53^tm1Brd/Trp53^tm1Brd; Tg(KRT14-rtTA)208Jek/Y; Tg(tetO-KLF4)32831Rup/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6NTac * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

integument

abnormal skin morphology ( J:96886 )

♂ • male animals exhibit epithelial dysplasia associated with infiltration of the dermis by well-vascularized, cellular or fibrotic stroma, or a fibrovascular response upon induction by doxycycline

Genotype
MGI:3717876

cx2

• Allelic Composition: Trp53^tm1Brd/Trp53⁺; Tg(KRT14-rtTA)208Jek/0; Tg(tetO-KLF4)32831Rup/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6NTac * SJL

integument

abnormal skin morphology ( J:96886 )

• females display focal changes including cystic follicles and mild changes in the adjacent interfollicular epithelium

Genotype
MGI:3717902

cx3

• Allelic Composition: Tg(KRT14-rtTA)208Jek/Y; Tg(tetO-KLF4)32831Rup/0
• Genetic Background: involves: C57BL/6 * SJL

integument

sebaceous gland atrophy ( J:96886 )

♂ • 9 days of doxycycline treatment to induce KLF4 expression, gland atrophy is observed

abnormal coat appearance ( J:96886 )

♂ • thinning of the pelage of the ventral skin is seen after 3-4 weeks of treatment with doxycycline

hair follicle degeneration ( J:96886 )

♂ • 9 days of doxycycline treatment to induce KLF4 expression, cystic degeneration of hair follicles is seen

abnormal skin morphology ( J:96886 )

♂ • in males, dysplastic phenotype is largely restricted to the ventral skin; dorsal skin is only mildly affected

♂ • females homozygous for Tg(KRT14-rtTA)208Jek display focal changes including cystic follicles and mild changes in the adjacent interfollicular epithelium after 4 weeks of induction

♂ • delayed squamous epithelium maturation is observed after 3-4 weeks of doxycycline treatment in males

abnormal epidermal layer morphology ( J:96886 )

♂ • hyperplastic, keratinizing epithelium extends into the dermis

abnormal epidermis stratum basale morphology ( J:96886 )

♂ • loss of basal cell polarity seen after 3-4 weeks of doxycycline treatment

hyperkeratosis ( J:96886 )

♂ • 9 days of doxycycline treatment to induce KLF4 expression, mouse skin shows hyperkeratosis

epidermal hyperplasia ( J:96886 )

♂ • 9 days of doxycycline treatment to induce KLF4 expression, epidermal hyperplasia is observed

skin lesions ( J:96886 )

♂ • crusted skin lesions are evident after 3-4 weeks of doxycycline treatment

endocrine/exocrine glands

sebaceous gland atrophy ( J:96886 )

♂ • 9 days of doxycycline treatment to induce KLF4 expression, gland atrophy is observed