Phenotypes associated with this allele

• Allele Symbol: Ins2^C95S
• Allele Name: C95S
• Allele ID: MGI:3713760
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ins2^C95S/Ins2^C95S	C3HeB/FeJ-Ins2^C95S	MGI:3714126
ht2	Ins2^C95S/Ins2^+	C3HeB/FeJ-Ins2^C95S	MGI:3714127

Genotype
MGI:3714126

hm1

• Allelic Composition: Ins2^C95S/Ins2^C95S
• Genetic Background: C3HeB/FeJ-Ins2^C95S

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:122098 )

• males and females die at a mean age of 46 days and 52 days, respectively

homeostasis/metabolism

increased circulating glucose level ( J:122098 )

• males and females have increased glucose levels at day 21

increased urine glucose level ( J:122098 )

• mice are normal until day 18 when they develop glucosuria and diabetes

immune system

increased susceptibility to autoimmune diabetes ( J:122098 )

• mice are normal until day 18 when they develop glucosuria and diabetes

renal/urinary system

increased urine glucose level ( J:122098 )

• mice are normal until day 18 when they develop glucosuria and diabetes

growth/size/body

decreased body weight ( J:122098 )

• by day 28, body weights of males and females are reduced

Genotype
MGI:3714127

ht2

• Allelic Composition: Ins2^C95S/Ins2⁺
• Genetic Background: C3HeB/FeJ-Ins2^C95S

homeostasis/metabolism

decreased insulin secretion ( J:122098 )

• pancreatic insulin levels are reduced

increased circulating glucose level ( J:122098 )

• 48% of males and 41% of females are considered diabetic (i.e. showing glucosuria or exhibiting blood glucose levels greater than 160mg/dl for females and 190mg/dl for males)

• at 1, 3, 6 months fasted and 1.5 hours postprandial blood glucose levels are elevated in males and females

• however, blood glucose levels at 3 weeks are normal

hyperglycemia ( J:122098 , J:137483 )

• in 66% of mice (J:137483)

decreased circulating insulin level ( J:122098 )

• at 10 minutes after glucose challenge, serum insulin levels are reduced

increased urine glucose level ( J:122098 )

• 48% of males and 41% of females are considered diabetic (i.e. showing glucosuria or exhibiting blood glucose levels greater than 160mg/dl for females and 190mg/dl for males)

insulin resistance ( J:122098 )

• homeostasis model assessment (HOMA) of insulin resistance is higher in 1 month old females and in 3 and 6 month old males

endocrine/exocrine glands

abnormal pancreatic islet morphology ( J:122098 )

• islet volume is significantly lower due to a decrease in beta cell volume while alpha, delta and PP cell volumes are significantly higher

• however, pancreatic volume is normal

increased pancreatic alpha cell number ( J:122098 )

• alpha cell numbers are increased

abnormal pancreatic beta cell morphology ( J:122098 )

• while no apoptotic bodies are observed rough endoplasmic reticulum is disorganized and mitochondria are swollen

• insulin secretory granules are almost missing and remaining granules are small

decreased pancreatic beta cell number ( J:122098 )

• insulin secretory granules are almost missing

disorganized pancreatic islets ( J:122098 )

• alpha cells are dispersed over the islet profile unlike in wild-type mice

abnormal pancreatic beta cell physiology ( J:122098 )

• homeostasis model assessment (HOMA) beta-cell index is reduced

decreased insulin secretion ( J:122098 )

• pancreatic insulin levels are reduced

immune system

increased susceptibility to autoimmune diabetes ( J:122098 )

• 48% of males and 41% of females are considered diabetic (i.e. showing glucosuria or exhibiting blood glucose levels greater than 160mg/dl for females and 190mg/dl for males)

renal/urinary system

increased urine glucose level ( J:122098 )

• 48% of males and 41% of females are considered diabetic (i.e. showing glucosuria or exhibiting blood glucose levels greater than 160mg/dl for females and 190mg/dl for males)

growth/size/body

decreased body weight ( J:122098 )

• fasted body weight at 3 and 6 months of ages for males is significantly lower than sex-matched wild-type mice