Phenotypes associated with this allele

• Allele Symbol: Ehd1^tm1Mhor
• Allele Name: targeted mutation 1, Mia Horowitz
• Allele ID: MGI:3712350
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ehd1^tm1Mhor/Ehd1^tm1Mhor	either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Swiss Webster)	MGI:3712748
hm2	Ehd1^tm1Mhor/Ehd1^tm1Mhor	involves: 129S6/SvEvTac	MGI:3712757

Genotype
MGI:3712748

hm1

• Allelic Composition: Ehd1^tm1Mhor/Ehd1^tm1Mhor
• Genetic Background: either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Swiss Webster)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

cellular phenotype ( J:121819 )

N • in primary mouse embryonic fibroblasts (MEFs), no differences in growth rate are detected compared to wild-type

abnormal cell physiology ( J:121819 )

• recycling to plasma membrane in MEFs is impaired with Ehd1 deficiency; disappearance of transferrin after a 1 hour pulse is attenuated relative to wild-type cells, with accumulation in perinuclear endocytic recycling-like structures in cells

Genotype
MGI:3712757

hm2

• Allelic Composition: Ehd1^tm1Mhor/Ehd1^tm1Mhor
• Genetic Background: involves: 129S6/SvEvTac

cellular

abnormal cell migration ( J:120764 )

• mouse embryonic fibroblasts (MEFs) plated on fibronectin show reduced migration and colonization to a scratch wound area; mutant fibroblasts colonize 15% of wound area compared to 65% of wound area by wild-type fibroblasts

decreased fibroblast cell migration ( J:120764 )

• Ehd1-null MEFs plated on fibronectin exhibit few filopodia extending toward the wound area compared to wild-type MEFs

• fibroblast rate of migration is reduced on fibronectin; mutant fibroblasts show a considerable delay in spreading on a fibroncectin-coated surface