Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc20Gt(XE368)Byg mutation
(0 available);
any
Cdc20 mutation
(30 available)
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mortality/aging
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• two-cell embryos are never recovered after E3.5
• 22% of E1.5 embryos cultured for 18 hours arrested at the two-cell stage
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• some embryos are found when analyzed at E7.5 (3/41); these are much smaller than littermates, and appear unlikely to develop further
• no homozygous embryos are recovered after E7.5
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cellular
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• chromosomes from E1.5 embryos cultured for 16 hours show arrest in metaphase; sister chromatides have not separated and arm cohesion remains intact
• metaphase to anaphase transition is blocked
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc20Gt(XE368)Byg mutation
(0 available);
any
Cdc20 mutation
(30 available)
Cdc20tm1Jvd mutation
(0 available);
any
Cdc20 mutation
(30 available)
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reproductive system
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N |
• male mice exhibit normal reproduction and normal levels of apoptosis in the testes
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• female mice produce more aneuploid oocytes than wild-type mice
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• primary and secondary spermatocytes are more prone to aneuploidy compared to in wild-type mice
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• average time from metaphase entry to anaphase is twice as long as in wild-type oocytes
• chromosomes are misaligned during meiosis I in oocyte compared to in wild-type mice
• however, timing of germinal vesicle breakdown is normal
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• 4 of 7 female mice fail to produce pups
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• female mice produce an average 4-fold fewer litters with an average 15-fold lower number of pups compared with wild-type mice
• however, mice exhibit normal oogenesis and fertilization
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cellular
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• female mice produce more aneuploid oocytes than wild-type mice
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• primary and secondary spermatocytes are more prone to aneuploidy compared to in wild-type mice
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• female mice produce more aneuploid oocytes than wild-type mice
• primary and secondary spermatocytes are more prone to aneuploidy compared to in wild-type mice
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• average time from metaphase entry to anaphase is twice as long as in wild-type oocytes
• chromosomes are misaligned during meiosis I in oocyte compared to in wild-type mice
• however, timing of germinal vesicle breakdown is normal
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• only 15% of embryos derived from dams form blastocysts compared with 93% of embryos collected from wild-type dams
• dams produce embryos that cease proliferating after the first cell division of preimplantation period and do not develop beyond the 4-cell stage unlike embryos from wild-type dams
• dams produce more aneuploid embryos compared with wild-type mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc20Gt(XE368)Byg mutation
(0 available);
any
Cdc20 mutation
(30 available)
Pttg1tm1Pzg mutation
(0 available);
any
Pttg1 mutation
(16 available)
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mortality/aging
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• embryos arrested at the two-cell stage can be isolated at E3.5
• analysis of E1.5 embryos cultured for 18 hours showed that some embryos cannot divide further and arrest at 2 -cell stage
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cellular
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• unlike Cdc20-null embryos, chromosomes do not display metaphase configuration, but are scattered; sister chromatids are separated
• in E1.5 cultured embryos, two-cell embryos stay in metaphase for ~4 hours, then begin to wander away from the metaphase plates
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Analysis Tools