Phenotypes associated with this allele

• Allele Symbol: Thbd^tm2.1Emc
• Allele Name: targeted mutation 2.1, Edward M Conway
• Allele ID: MGI:3698840
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Thbd^tm2.1Emc/Thbd^tm2.1Emc	involves: 129/Sv * BALB/c * C57BL/6	MGI:3712461

Genotype
MGI:3712461

hm1

• Allelic Composition: Thbd^tm2.1Emc/Thbd^tm2.1Emc
• Genetic Background: involves: 129/Sv * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal leukocyte physiology ( J:118000 )

• leukocyte accumulation in the lung is increased

• inhaled LPS results in 3.5-fold higher bronchoalveolar lavage myeloperoxidase activity and 2-fold higher polymorphonuclear leukocyte counts

• polymorphonuclear leukocytes found in the area at risk following myocardial ischemia and reperfusion are increased 2.5-fold and 4.8-fold in the right and left ventricle, respectively

• extravasation of polymorphonuclear leukocytes following myocardial ischemia and reperfusion is increased

abnormal leukocyte adhesion ( J:118000 )

• permanent adhesion of polymorphonuclear leukocytes to mutant endothelial cells is increased 7.8-fold compared to wild-type endothelial cells

• TNFA-induced increase in adhesion is 3.1-fold greater assays using mutant endothelial cells compared to assays using wild-type cells

• however, mutant polymorphonuclear leukocyte rolling and adhesion are not significantly different from wild-type when compared using the same endothelial cells

increased circulating interleukin-1 beta level ( J:118000 )

• 6 hours after injection of 20ug/g LPS levels in serum of IL-1B are significantly higher compared to wild-type mice

increased circulating tumor necrosis factor level ( J:118000 )

• 6 hours after injection of 20ug/g LPS levels of TNF-alpha in serum are significantly higher compared to wild-type mice

increased susceptibility to endotoxin shock ( J:118000 )

• following intraperitoneal injection of 40 ug/g LPS greater than 50% of mice die within 26 hours compared to less than 10% of wild-type mice

• 6 hours after LPS injection (20 ug/g), TNFA and IL-1B levels are significantly increased compared to wild-type mice

• inhaled LPS results in enhanced bronchoalveolar lavage myeloperoxidase activty (3.5-fold) and increased polymorphonuclear leukocyte counts (2-fold)

cardiovascular system

increased myocardial infarct size ( J:118000 )

• infarction size is increased following myocardial ischemia and reperfusion as a function of either left ventricle area or area at risk

homeostasis/metabolism

increased myocardial infarct size ( J:118000 )

• infarction size is increased following myocardial ischemia and reperfusion as a function of either left ventricle area or area at risk

increased circulating interleukin-1 beta level ( J:118000 )

• 6 hours after injection of 20ug/g LPS levels in serum of IL-1B are significantly higher compared to wild-type mice

increased circulating tumor necrosis factor level ( J:118000 )

• 6 hours after injection of 20ug/g LPS levels of TNF-alpha in serum are significantly higher compared to wild-type mice

cellular

abnormal leukocyte adhesion ( J:118000 )

• permanent adhesion of polymorphonuclear leukocytes to mutant endothelial cells is increased 7.8-fold compared to wild-type endothelial cells

• TNFA-induced increase in adhesion is 3.1-fold greater assays using mutant endothelial cells compared to assays using wild-type cells

• however, mutant polymorphonuclear leukocyte rolling and adhesion are not significantly different from wild-type when compared using the same endothelial cells

hematopoietic system

abnormal leukocyte physiology ( J:118000 )

• leukocyte accumulation in the lung is increased

• inhaled LPS results in 3.5-fold higher bronchoalveolar lavage myeloperoxidase activity and 2-fold higher polymorphonuclear leukocyte counts

• polymorphonuclear leukocytes found in the area at risk following myocardial ischemia and reperfusion are increased 2.5-fold and 4.8-fold in the right and left ventricle, respectively

• extravasation of polymorphonuclear leukocytes following myocardial ischemia and reperfusion is increased

abnormal leukocyte adhesion ( J:118000 )

• permanent adhesion of polymorphonuclear leukocytes to mutant endothelial cells is increased 7.8-fold compared to wild-type endothelial cells

• TNFA-induced increase in adhesion is 3.1-fold greater assays using mutant endothelial cells compared to assays using wild-type cells

• however, mutant polymorphonuclear leukocyte rolling and adhesion are not significantly different from wild-type when compared using the same endothelial cells