Phenotypes associated with this allele

• Allele Symbol: Tg(GNAT2-Dta)98Wwk
• Allele Name: transgene insertion 98, Winston W-Y Kao
• Allele ID: MGI:3693959
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(GNAT2-Dta)98Wwk/0	involves: C57BL/6J * FVB/N	MGI:3694080

Genotype
MGI:3694080

tg1

• Allelic Composition: Tg(GNAT2-Dta)98Wwk/0
• Genetic Background: involves: C57BL/6J * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

gliosis ( J:102232 )

• a gliosis marker is detected in ventral portion spanning the retina

abnormal retina photoreceptor morphology ( J:102232 )

• photoreceptors are noticeably absent in ventral portion of retina

• photoreceptor shape becomes more distorted in ventral region of transitional zone

• partial and malformed cells are still present in transitional zone when normal retinal cells reach maturity (P12-P23), and persist in older animals

absent retina cone cells ( J:102232 )

• at P23 and P60, cone photoreceptors are not distinguishable in rod population

decreased retina rod cell number ( J:102232 )

• at P23, rods are found only in dorsal half of mutant retina

absent retina rod cells ( J:102232 )

• rods are typically absent in most of ventral retina at P60

abnormal photoreceptor inner segment morphology ( J:102232 )

• thinning of inner segment layer (ISL) accompanies reduction in photoreceptors

• in photoreceptors in ventral region of transitional zone, length of inner segment becomes steadily attenuated, such that remaining cells have truncated and thickened inner segment with distorted shapes

abnormal photoreceptor outer segment morphology ( J:102232 , J:116305 )

• thinning of outer segment layer (OSL) accompanies reduction in photoreceptors at P23 (J:102232)

• in photoreceptors in ventral region of transitional zone, length of inner segment becomes steadily attenuated (J:102232)

• thinner than in wild-type (J:116305)

retina photoreceptor degeneration ( J:116305 )

• number of photoreceptors decreases with age

retina cone cell degeneration ( J:116305 )

• retina begins to lose cone cells at P8, and at P21, cone cells are absent among remaining photoreceptor cells

retina rod cell degeneration ( J:116305 )

• age-related rod degeneration begins at P9, and proceeds from central retina to peripheral retina

vision/eye

abnormal retina morphology ( J:102232 )

• middle portion of transgenic retinal contains transitional zone extending dorsally and ventrally from the optic disk; in outer nuclear layer (ONL) of this zone, photoreceptor nuclei decrease

• retinal becomes thinner in more ventral regions at P23, containing fewer cellular and synaptic layers

• at P8, immature photorecptor cells with partially formed inner segments are segregated from neurons in a newly-formed inner nuclear layer (INL); a synaptic outer plexiform layer is found between these layers; in ventral retina, such differentiation has not occurred

• cells with small rounded somas and lacking segmentation line distal inner nuclear layer/RPE junction

• by P30-68, radial thickness of dorsal retina decreases to ~80-100 um, while ventral region is dramatically thinner (~45-55 um) compared to wild-type retina radial thickness of 110-130 um

• by P360, dorsal retina is 70-100 um thick, while ventral region remains 45-55 um

abnormal retina photoreceptor morphology ( J:102232 )

• photoreceptors are noticeably absent in ventral portion of retina

• photoreceptor shape becomes more distorted in ventral region of transitional zone

• partial and malformed cells are still present in transitional zone when normal retinal cells reach maturity (P12-P23), and persist in older animals

absent retina cone cells ( J:102232 )

• at P23 and P60, cone photoreceptors are not distinguishable in rod population

decreased retina rod cell number ( J:102232 )

• at P23, rods are found only in dorsal half of mutant retina

absent retina rod cells ( J:102232 )

• rods are typically absent in most of ventral retina at P60

abnormal photoreceptor inner segment morphology ( J:102232 )

• thinning of inner segment layer (ISL) accompanies reduction in photoreceptors

• in photoreceptors in ventral region of transitional zone, length of inner segment becomes steadily attenuated, such that remaining cells have truncated and thickened inner segment with distorted shapes

abnormal photoreceptor outer segment morphology ( J:102232 , J:116305 )

• thinning of outer segment layer (OSL) accompanies reduction in photoreceptors at P23 (J:102232)

• in photoreceptors in ventral region of transitional zone, length of inner segment becomes steadily attenuated (J:102232)

• thinner than in wild-type (J:116305)

retina photoreceptor degeneration ( J:116305 )

• number of photoreceptors decreases with age

retina cone cell degeneration ( J:116305 )

• retina begins to lose cone cells at P8, and at P21, cone cells are absent among remaining photoreceptor cells

retina rod cell degeneration ( J:116305 )

• age-related rod degeneration begins at P9, and proceeds from central retina to peripheral retina

thin retina outer nuclear layer ( J:102232 , J:116305 )

• at P30-68, 8-10 rows of nuclei are present in dorsal ONL, compared to 10-12 in dorsal and ventral wild-type retina (J:102232)

• by P360, there are 7-8 rows of nuclei in ONL (J:102232)

• in transitional zone, photoreceptor nuclei in ONL decreases from 8-9 rows dorsally to 0 ventrally (J:102232)

• at P9, outer nuclear layer (ONL) of central retina is thinner than in wild-type; ONL is 3-4 layer thick compared to 9-10 in wild-type (J:116305)

• at P30, ONL of central retina consists of only one layer of photoreceptor cell bodies; in peripheral retina, ONL contains 2-3 layers of cell bodies (J:116305)

• at P60, entire ONL at very center of retina has disappeared; in peripheral retina, only one cell layer remains (J:116305)

• at P180, in central and peripheral regions in ventral portion of retina, ONL is absent (J:116305)

thin retina outer plexiform layer ( J:102232 )

• outer plexiform layer (OPL) is thinner