Phenotypes associated with this allele

• Allele Symbol: Six3^tm2.1Gco
• Allele Name: targeted mutation 2.1, Guillermo Oliver
• Allele ID: MGI:3693322
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Six3^tm2.1Gco/Six3^tm2.1Gco	involves: 129S1/Sv	MGI:3693846
cn2	Six3^tm2Gco/Six3^tm2.1Gco Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S1/Sv * C57BL/6 * CBA	MGI:3693848
cn3	Six3^tm2Gco/Six3^tm2.1Gco Tg(Pax6-cre,GFP)1Pgr/0	involves: 129S1/Sv * FVB/N	MGI:3693847

Genotype
MGI:3693846

hm1

• Allelic Composition: Six3^tm2.1Gco/Six3^tm2.1Gco
• Genetic Background: involves: 129S1/Sv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal forebrain development ( J:116102 )

decreased forebrain size ( J:116102 )

• prosencephalon is truncated

Genotype
MGI:3693848

cn2

• Allelic Composition: Six3^tm2Gco/Six3^tm2.1Gco; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129S1/Sv * C57BL/6 * CBA

vision/eye

abnormal lens morphology ( J:116102 )

abnormal lens development ( J:116102 )

• defects are observed starting at E10.5

• reduction in size of invaginating lens pit is observed in mildly and moderately affected embryos

• at E14.5, some embryos have an abnormally persistent lens stalk

• differentiation of lens is not affected in mildly or moderately affected mutant lenses

abnormal lens induction ( J:116102 )

• in severely affected lenses at E10.5, no lens-like structures are present

abnormal lens vesicle development ( J:116102 )

• at E12.5, in mild cases, lens vesicle is small but relatively normal, small and abnormal in moderated cases and completely absent in severely affected embryos and neuroretinal is malformed also

abnormal lens fiber morphology ( J:116102 )

• at E14.5 some embryos show disorganized lens fibers

aphakia ( J:116102 )

• lens is absent in some mutants

cataract ( J:116102 )

• cataracts are present in some mice

small lens ( J:116102 )

• some mice show drastically reduced lens size

abnormal optic vesicle formation ( J:116102 )

• shape of vesicle is defective

Genotype
MGI:3693847

cn3

• Allelic Composition: Six3^tm2Gco/Six3^tm2.1Gco; Tg(Pax6-cre,GFP)1Pgr/0
• Genetic Background: involves: 129S1/Sv * FVB/N

nervous system

abnormal forebrain development ( J:116102 )

• some embryos injected with tamoxifen early show defective forebrain patterning

vision/eye

abnormal lens induction ( J:116102 )

• when tamoxifen is given at E7.5 and 8.5, no thickening or invagination of the PLE is observed at least in one side