Phenotypes associated with this allele

• Allele Symbol: Nit1^tm1Hbn
• Allele Name: targeted mutation 1, Kay Huebner
• Allele ID: MGI:3691030
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Nit1^tm1Hbn/Nit1^tm1.1Hbn	involves: 129X1/SvJ * C57BL/6	MGI:3691216
cn2	Nit1^tm1Hbn/Nit1^tm1Hbn	involves: 129X1/SvJ * C57BL/6	MGI:3691218
cn3	Nit1^tm1Hbn/Nit1^tm1Hbn Tg(Wap-cre)11738Mam/0	involves: 129X1/SvJ * C57BL/6 * SJL	MGI:3691220

Genotype
MGI:3691216

ht1

• Allelic Composition: Nit1^tm1Hbn/Nit1^tm1.1Hbn
• Genetic Background: involves: 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased incidence of tumors by chemical induction ( J:115052 )

• mice receiving 6 intragastric doses of N-nitrosomethylbenzylamine (NMBA) over 3 weeks show increased incidence of forestomach papillomas and dysplasias (carcinomas in situ) compared to controls; number of papillomas and dysplasias is ~equivalent to numbers in Fhit-deficient mice

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:115052 )

• mice receiving 6 intragastric doses of N-nitrosomethylbenzylamine (NMBA) over 3 weeks show increased incidence of forestomach papillomas and dysplasias (carcinomas in situ) compared to controls; number of papillomas and dysplasias is ~equivalent to numbers in Fhit-deficient mice

Genotype
MGI:3691218

cn2

• Allelic Composition: Nit1^tm1Hbn/Nit1^tm1Hbn
• Genetic Background: involves: 129X1/SvJ * C57BL/6

cellular

abnormal cell morphology ( J:115052 )

• cells are transfected with a retroviral cre to produce Nit1-deficient cells; Nit1-null cells have round and compact shapes, loss of lobular structure, higher cell density with increased S and G2/M cell populations, and >3-fold higher clonogenicity compared to cells not treated with cre recombinase

decreased cellular sensitivity to gamma-irradiation ( J:115052 )

• after treatment with mitomycin C (MMC) or UV-C light, Nit1-deficient cells show enhanced survival compared to Nit1-sufficient cells

Genotype
MGI:3691220

cn3

• Allelic Composition: Nit1^tm1Hbn/Nit1^tm1Hbn; Tg(Wap-cre)11738Mam/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * SJL

endocrine/exocrine glands

abnormal branching of the mammary ductal tree ( J:115052 )

♀ • there is only a small significant difference in number of branch points during pregnancy

♀ • at mid-pregnant stages (day13 and 16), number of endbuds is significantly increased compared to controls

♀ • null mammary glands show an increase in ductal structures; lobuloalveolar structures are better developed at later times during pregnancy compared to controls

integument

abnormal branching of the mammary ductal tree ( J:115052 )

♀ • there is only a small significant difference in number of branch points during pregnancy

♀ • at mid-pregnant stages (day13 and 16), number of endbuds is significantly increased compared to controls

♀ • null mammary glands show an increase in ductal structures; lobuloalveolar structures are better developed at later times during pregnancy compared to controls