Phenotypes associated with this allele

• Allele Symbol: Serpinb9^tm1Arp
• Allele Name: targeted mutation 1, Philip G Ashton-Rickardt
• Allele ID: MGI:3687210
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Serpinb9^tm1Arp/Serpinb9^tm1Arp	involves: C57BL/6	MGI:3687312
cx2	Gzmb^tm1Ley/Gzmb^tm1Ley Serpinb9^tm1Arp/Serpinb9^tm1Arp	involves: 129S2/SvPas * C57BL/6	MGI:3687315
cx3	Serpinb9^tm1Arp/Serpinb9^tm1Arp Tg(TcrLCMV)327Sdz/0	involves: C57BL/6	MGI:3687316

Genotype
MGI:3687312

hm1

• Allelic Composition: Serpinb9^tm1Arp/Serpinb9^tm1Arp
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

increased apoptosis ( J:113349 )

• apoptosis is increased in LCMV- and LM-specific CTLs in mutants

hematopoietic system

abnormal CD8-positive, alpha-beta cytotoxic T cell morphology ( J:113349 )

• upon infection with LCMV Armstrong or a strain of Listeria monocytogenes (LM), numbers of LCMV-specific CTLs are reduced 5-fold and LM-specific CTLs are reduced 9-fold compared to control B6 mice

• frequency of CTLs devoid of Gsmb granules is 3 times greater than in controls; number of Gzmb granules per CTL is 2-fold lower than in controls

abnormal cytotoxic T cell physiology ( J:113349 )

• apoptosis is increased in LCMV- and LM-specific CTLs in mutants

decreased cytotoxic T cell cytolysis ( J:113349 )

• deficit in CTL activity is observed in ex vivo activity assay over course of LCMV infection; on day 10, activity is decrease 6-fold compared to controls

homeostasis/metabolism

abnormal enzyme/coenzyme level ( J:113349 )

• caspase 3 in cytoplasm is increased

abnormal enzyme/coenzyme activity ( J:113349 )

• GranzymeB activity is significantly increased in cytoplasm of mutants compared to controls

immune system

abnormal CD8-positive, alpha-beta cytotoxic T cell morphology ( J:113349 )

• upon infection with LCMV Armstrong or a strain of Listeria monocytogenes (LM), numbers of LCMV-specific CTLs are reduced 5-fold and LM-specific CTLs are reduced 9-fold compared to control B6 mice

• frequency of CTLs devoid of Gsmb granules is 3 times greater than in controls; number of Gzmb granules per CTL is 2-fold lower than in controls

abnormal cytotoxic T cell physiology ( J:113349 )

• apoptosis is increased in LCMV- and LM-specific CTLs in mutants

decreased cytotoxic T cell cytolysis ( J:113349 )

• deficit in CTL activity is observed in ex vivo activity assay over course of LCMV infection; on day 10, activity is decrease 6-fold compared to controls

increased susceptibility to Riboviria infection ( J:113349 )

• clearance of LCMV is defective in mutants; titer of virus is 10-fold higher on day 6 of infection compared to wild-type

Genotype
MGI:3687315

cx2

• Allelic Composition: Gzmb^tm1Ley/Gzmb^tm1Ley; Serpinb9^tm1Arp/Serpinb9^tm1Arp
• Genetic Background: involves: 129S2/SvPas * C57BL/6

immune system

abnormal CD8-positive, alpha-beta cytotoxic T cell morphology ( J:113349 )

• after LCMV infection, numbers of LCMV-specific CTLs are same as wild-type

abnormal cytotoxic T cell physiology ( J:113349 )

• level of apoptosis of LCMV-specific CTLs in mutants is same as in wild-type compared to increase seen in single knockouts

hematopoietic system

abnormal CD8-positive, alpha-beta cytotoxic T cell morphology ( J:113349 )

• after LCMV infection, numbers of LCMV-specific CTLs are same as wild-type

abnormal cytotoxic T cell physiology ( J:113349 )

• level of apoptosis of LCMV-specific CTLs in mutants is same as in wild-type compared to increase seen in single knockouts

Genotype
MGI:3687316

cx3

• Allelic Composition: Serpinb9^tm1Arp/Serpinb9^tm1Arp; Tg(TcrLCMV)327Sdz/0
• Genetic Background: involves: C57BL/6

immune system

abnormal response to transplant ( J:113349 )

• when naive CD8 T cells transferred into B6 Ptprca recipients that were then infected with LCMV, numbers of viral-specific CTLs are reduced 7-fold compared to recipients receiving wild-type transgenic T cells