Phenotypes associated with this allele

• Allele Symbol: Arhgef1^tm1.1Rmt
• Allele Name: targeted mutation 1.1, Raul M Torres
• Allele ID: MGI:3686863
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Arhgef1^tm1.1Rmt/Arhgef1^tm1.1Rmt	C57BL/6-Arhgef1^tm1.1Rmt	MGI:3687247
cx2	Igh^tm2Cgn/Igh^+ Rr158^tm1Cgn/Rr158^tm1Cgn Arhgef1^tm1.1Rmt/Arhgef1^tm1.1Rmt	involves: 129P2/OlaHsd * C57BL/6	MGI:3687248

Genotype
MGI:3687247

hm1

• Allelic Composition: Arhgef1^tm1.1Rmt/Arhgef1^tm1.1Rmt
• Genetic Background: C57BL/6-Arhgef1^tm1.1Rmt

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased B cell number ( J:113295 )

• variable decrease in peripheral B cells from 20% in lymph nodes to 50% in the spleen

decreased B-1 B cell number ( J:113295 )

• 80% decrease in peritoneal B1 cells

decreased follicular B cell number ( J:113295 )

• about a 50% reduction in follicular B cells in spleen

decreased marginal zone B cell number ( J:113295 )

• about a 50% reduction in marginal zone B cells in spleen

decreased IgM level ( J:113295 )

• increase in response to T cell dependent antigen stimulation is less than 20% of wild-type; however unstimulated levels of IgM and IgG1 are similar to wild-type

• no difference is seen in antigen specific IgM response between sham-treated and asplenic mice, unlike in wild-type mice where removal of the spleen significantly reduces antigen specific IgM response

abnormal marginal zone B cell physiology ( J:113295 )

• prolonged adhesion of marginal zone B cells to integrin-ligands both in vitro and in vivo

• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate concentrations

• 24 hours after immunization with LPS or LPS plus T cell dependent antigen (4-hydroxy-3-mitrophenylacetyl-chicken gamma-globulin, NP-CG) a significant fraction of B cells remain in the marginal zone unlike in wild-type mice

hematopoietic system

decreased B cell number ( J:113295 )

• variable decrease in peripheral B cells from 20% in lymph nodes to 50% in the spleen

decreased B-1 B cell number ( J:113295 )

• 80% decrease in peritoneal B1 cells

decreased follicular B cell number ( J:113295 )

• about a 50% reduction in follicular B cells in spleen

decreased marginal zone B cell number ( J:113295 )

• about a 50% reduction in marginal zone B cells in spleen

decreased IgM level ( J:113295 )

• increase in response to T cell dependent antigen stimulation is less than 20% of wild-type; however unstimulated levels of IgM and IgG1 are similar to wild-type

• no difference is seen in antigen specific IgM response between sham-treated and asplenic mice, unlike in wild-type mice where removal of the spleen significantly reduces antigen specific IgM response

abnormal marginal zone B cell physiology ( J:113295 )

• prolonged adhesion of marginal zone B cells to integrin-ligands both in vitro and in vivo

• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate concentrations

• 24 hours after immunization with LPS or LPS plus T cell dependent antigen (4-hydroxy-3-mitrophenylacetyl-chicken gamma-globulin, NP-CG) a significant fraction of B cells remain in the marginal zone unlike in wild-type mice

Genotype
MGI:3687248

cx2

• Allelic Composition: Igh^tm2Cgn/Igh⁺; Rr158^tm1Cgn/Rr158^tm1Cgn; Arhgef1^tm1.1Rmt/Arhgef1^tm1.1Rmt
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal B cell physiology ( J:113295 )

• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate (S1P) concentrations

• reduction in migratory response towards S1P induced by LPS treatment is decreased compared to wild-type

• immunization with a low dose of T cell dependent antigen (NP-CG) fails to suppress responsiveness to S1P unlike in wild-type mice; however, treatment with a high concentration of antigen does reduce S1P responsiveness

hematopoietic system

abnormal B cell physiology ( J:113295 )

• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate (S1P) concentrations

• reduction in migratory response towards S1P induced by LPS treatment is decreased compared to wild-type

• immunization with a low dose of T cell dependent antigen (NP-CG) fails to suppress responsiveness to S1P unlike in wild-type mice; however, treatment with a high concentration of antigen does reduce S1P responsiveness