Phenotypes associated with this allele

• Allele Symbol: Coro1a^tm1Achn
• Allele Name: targeted mutation 1, Andrew C Chan
• Allele ID: MGI:3664599
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Coro1a^tm1Achn/Coro1a^tm1Achn	B6.129X1-Coro1a^tm1Achn	MGI:3818533
hm2	Coro1a^tm1Achn/Coro1a^tm1Achn	involves: 129X1/SvJ	MGI:5302404
hm3	Coro1a^tm1Achn/Coro1a^tm1Achn	involves: 129X1/SvJ * C57BL/6	MGI:3706904
ht4	Coro1a^ptcd/Coro1a^tm1Achn	B6.Cg-Coro1a^ptcd Coro1a^tm1Achn	MGI:3818534
cx5	Coro1a^tm1Achn/Coro1a^tm1Achn Coro1b^tm1.1Nfoe/Coro1b^tm1.1Nfoe	involves: 129X1/SvJ * C57BL/6N	MGI:5302403

Genotype
MGI:3818533

hm1

• Allelic Composition: Coro1a^tm1Achn/Coro1a^tm1Achn
• Genetic Background: B6.129X1-Coro1a^tm1Achn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell apoptosis ( J:141431 )

decreased thymocyte number ( J:141431 )

• the percentage of mature (CD69^loCD62L^hi) single-positive thymocytes is decreased in these mice

hematopoietic system

increased T cell apoptosis ( J:141431 )

decreased thymocyte number ( J:141431 )

• the percentage of mature (CD69^loCD62L^hi) single-positive thymocytes is decreased in these mice

cellular

increased T cell apoptosis ( J:141431 )

endocrine/exocrine glands

decreased thymocyte number ( J:141431 )

• the percentage of mature (CD69^loCD62L^hi) single-positive thymocytes is decreased in these mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive		DOID:0090014	OMIM:608971	J:141431

Genotype
MGI:5302404

hm2

• Allelic Composition: Coro1a^tm1Achn/Coro1a^tm1Achn
• Genetic Background: involves: 129X1/SvJ

immune system

increased mast cell degranulation ( J:177785 )

decreased interleukin-6 secretion ( J:177785 )

• from bone marrow-derived mast cells stimulated with DNP-HSA

• however, cellular production is normal

decreased tumor necrosis factor secretion ( J:177785 )

• from bone marrow-derived mast cells stimulated with DNP-HSA

• however, cellular production is normal

hematopoietic system

increased mast cell degranulation ( J:177785 )

cellular

increased mast cell degranulation ( J:177785 )

Genotype
MGI:3706904

hm3

• Allelic Composition: Coro1a^tm1Achn/Coro1a^tm1Achn
• Genetic Background: involves: 129X1/SvJ * C57BL/6

immune system

abnormal leukocyte migration ( J:111593 )

• CD4+ T cells display defective chemotactic migration in response to chemokines including CCL19, CXCL12, and CCL25 in a transwell migration assay

• when adoptively transferred, CD4+ cells that are Coro1a-deficient display defective homing to lymph nodes (~60% decrease) compared to wild-type CD4+ cells

decreased T cell number ( J:111593 )

• there is a paucity of T cells in spleen and lymph nodes of mutants

• naive splenic T cells are decreased in number

• naive T cells are reduced in blood and lymph nodes

decreased CD4-positive, alpha-beta T cell number ( J:111593 )

• cell number is decreased in the blood, spleen, and lymph nodes

• small reduction to CD4+ thymocytes is observed

decreased CD8-positive, alpha-beta T cell number ( J:111593 )

• cell number is decreased in the blood, spleen, and lymph nodes

• small reduction to CD4+ thymocytes is observed

decreased memory T cell number ( J:111593 )

• number of memory/effector T cells are reduced in blood and lymph nodes

hematopoietic system

abnormal leukocyte migration ( J:111593 )

• CD4+ T cells display defective chemotactic migration in response to chemokines including CCL19, CXCL12, and CCL25 in a transwell migration assay

• when adoptively transferred, CD4+ cells that are Coro1a-deficient display defective homing to lymph nodes (~60% decrease) compared to wild-type CD4+ cells

decreased T cell number ( J:111593 )

• there is a paucity of T cells in spleen and lymph nodes of mutants

• naive splenic T cells are decreased in number

• naive T cells are reduced in blood and lymph nodes

decreased CD4-positive, alpha-beta T cell number ( J:111593 )

• cell number is decreased in the blood, spleen, and lymph nodes

• small reduction to CD4+ thymocytes is observed

decreased CD8-positive, alpha-beta T cell number ( J:111593 )

• cell number is decreased in the blood, spleen, and lymph nodes

• small reduction to CD4+ thymocytes is observed

decreased memory T cell number ( J:111593 )

• number of memory/effector T cells are reduced in blood and lymph nodes

cellular

abnormal leukocyte migration ( J:111593 )

• CD4+ T cells display defective chemotactic migration in response to chemokines including CCL19, CXCL12, and CCL25 in a transwell migration assay

• when adoptively transferred, CD4+ cells that are Coro1a-deficient display defective homing to lymph nodes (~60% decrease) compared to wild-type CD4+ cells

Genotype
MGI:3818534

ht4

• Allelic Composition: Coro1a^ptcd/Coro1a^tm1Achn
• Genetic Background: B6.Cg-Coro1a^ptcd Coro1a^tm1Achn

immune system

increased thymocyte number ( J:141431 )

• the percentage of mature (CD69^loCD62L^hi) single-positive thymocytes is increased in these mice

abnormal effector T cell morphology ( J:141431 )

• migrating T cells have irregularly shaped protrusions that are often larger in size than controls

decreased CD4-positive, alpha-beta T cell number ( J:141431 )

• CD4⁺ T cell numbers in the periphery are significantly decreased around 5-fold

decreased CD8-positive, alpha-beta T cell number ( J:141431 )

• CD8⁺ T cell numbers in the periphery are significantly decreased

abnormal T cell physiology ( J:141431 )

• T cells have defects in migrating on ICAM-coated surfaces

abnormal T cell activation ( J:141431 )

• upon CD3 activation, T cells flux less Ca²⁺ than wild-type controls

• however, similar Ca²⁺ flux results as controls are observed when CD4 stimulation is included

hematopoietic system

increased thymocyte number ( J:141431 )

• the percentage of mature (CD69^loCD62L^hi) single-positive thymocytes is increased in these mice

abnormal effector T cell morphology ( J:141431 )

• migrating T cells have irregularly shaped protrusions that are often larger in size than controls

decreased CD4-positive, alpha-beta T cell number ( J:141431 )

• CD4⁺ T cell numbers in the periphery are significantly decreased around 5-fold

decreased CD8-positive, alpha-beta T cell number ( J:141431 )

• CD8⁺ T cell numbers in the periphery are significantly decreased

abnormal T cell physiology ( J:141431 )

• T cells have defects in migrating on ICAM-coated surfaces

abnormal T cell activation ( J:141431 )

• upon CD3 activation, T cells flux less Ca²⁺ than wild-type controls

• however, similar Ca²⁺ flux results as controls are observed when CD4 stimulation is included

endocrine/exocrine glands

increased thymocyte number ( J:141431 )

• the percentage of mature (CD69^loCD62L^hi) single-positive thymocytes is increased in these mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive		DOID:0090014	OMIM:608971	J:141431

Genotype
MGI:5302403

cx5

• Allelic Composition: Coro1a^tm1Achn/Coro1a^tm1Achn; Coro1b^tm1.1Nfoe/Coro1b^tm1.1Nfoe
• Genetic Background: involves: 129X1/SvJ * C57BL/6N

immune system

increased mast cell degranulation ( J:177785 )

• more severe than in Coro1a^tm1Achn homozygotes

increased susceptibility to type I hypersensitivity reaction ( J:177785 )

• increased passive cutaneous anaphylaxis

decreased interleukin-6 secretion ( J:177785 )

• from bone marrow-derived mast cells stimulated with DNP-HSA as in Coro1a^tm1Achn homozygotes

• however, cellular production is normal

decreased tumor necrosis factor secretion ( J:177785 )

• from bone marrow-derived mast cells stimulated with DNP-HSA as in Coro1a^tm1Achn homozygotes

• however, cellular production is normal

hematopoietic system

increased mast cell degranulation ( J:177785 )

• more severe than in Coro1a^tm1Achn homozygotes

cellular

increased mast cell degranulation ( J:177785 )

• more severe than in Coro1a^tm1Achn homozygotes