All Phenotypes Klf2<tm1Jml> MGI Mouse

embryonic lethality during organogenesis, complete penetrance ( J:44352 )

• begin to die around E12.5 with all dead by E14.5

abnormal blood vessel morphology ( J:44352 )

• in umbilical vessels the tunica media is severely reduced in thickness and this reduction is patchy in umbilical arteries but more uniform in umbilical veins

• the tunica media of umbilical vessels also contains significantly less extracellular matrix compared to controls

abnormal aorta tunica media morphology ( J:44352 )

• the tunica media is poorly organized with vascular smooth muscle cells that have a cuboidal shape

abnormal vascular endothelial cell morphology ( J:44352 )

• some vascular endothelial cells in umbilical veins appear enlarged and necrotic

abnormal vascular smooth muscle morphology ( J:44352 )

• marked reduction in the number of cells in the tunica media of umbilical vessels

• cells in the tunica media of the aorta have a cuboidal morphology

aneurysm ( J:44352 )

• multiple aneurysmal dilations are seen in both umbilical arteries and veins and in many cases dissection of these vessels and associated hemorrhages are seen at the sites of dilation

hemorrhage ( J:44352 )

• by E13.5 nearly all embryos show intra-amniotic and/or intra-embryonic hemorrhage

• by E14.5 embryos have exsanguinated into the amniotic cavity

internal hemorrhage ( J:44352 )

• seen in 30% at E12.5 especially around the cardiac outflow tract and within the abdomen

anemia ( J:44352 )

• secondary to hemorrhage

• however, fetal liver cells produce normal numbers of erythroid and mixed colonies indicating normal fetal liver hematopoiesis

decreased embryo size ( J:44352 )

• at E13.5

abnormal embryonic tissue morphology ( J:44352 )

• by E13.5 embryos appear bloated with pale heads and limbs and widespread necrosis

embryo tissue necrosis ( J:44352 )

• widespread necrosis by E13.5, secondary to hemorrhage