Phenotypes associated with this allele

• Allele Symbol: Fezf1^tm1Hibi
• Allele Name: targeted mutation 1, Masahiko Hibi
• Allele ID: MGI:3626294
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fezf1^tm1Hibi/Fezf1^tm1Hibi	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)	MGI:3628943
cx2	Fezf1^tm1Hibi/Fezf1^tm1Hibi Fezf2^tm1Hibi/Fezf2^tm1Hibi	involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3767818
cx3	Fezf1^tm1Hibi/Fezf1^tm1Hibi Fezf2^tm1Hibi/Fezf2^+	involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3767819

Genotype
MGI:3628943

hm1

• Allelic Composition: Fezf1^tm1Hibi/Fezf1^tm1Hibi
• Genetic Background: either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, incomplete penetrance ( J:107405 )

• most die within 1 day of birth

nervous system

abnormal neuronal migration ( J:107405 )

• at E16.5 the number of proliferating cells is decreased in the subventricular zone and at E18.5 no BrdU labelled cells are seen in the glomerular layer suggesting that migration of interneurons into the glomerular layer is impaired

abnormal olfactory sensory neuron morphology ( J:107405 )

• olfactory axons pass through the lamina cribosa by E12.5 but never reach the olfactory bulb and instead form a fibrocellular mass outside the olfactory bulb

• however, expression analysis indicates that differentiation of olfactory neurons is not significantly different from wild-type

abnormal olfactory bulb development ( J:107405 )

• olfactory axons pass through the lamina cribosa by E12.5 but never reach the olfactory bulb and instead form a fibrocellular mass outside the olfactory bulb

• at E16.5 the number of proliferating cells is decreased in the subventricular zone and at E18.5 no BrdU labelled cells are seen in the glomerular layer suggesting that migration of interneurons into the glomerular layer is impaired

• at E18.5 the olfactory sensory nerve layer (ONL) is absent and ensheathing cells normally found in the ONL are detected in the fibrocellular mass

• at E18.5, the mitral cell layer, now the outer most layer, is thicker than normal and the polarity of the mitral cells is disorganized

• at E18.5, the glomerular layer fails to form and cells expressing of Dlx1, Gad67, and Th are disorganized

abnormal rostral migratory stream morphology ( J:107405 )

• at E15.5 and E18.5 the rostral migratory stream is shorter and wider with a disorganized anterior tip also present at E18.5

small olfactory bulb ( J:107405 )

• seen at E18.5 and P0; however telencephalon and diencephalon morphology are similar to wild-type

abnormal embryonic/fetal subventricular zone morphology ( J:107405 )

• at E16.5 the number of proliferating cells is decreased in the subventricular zone

cellular

abnormal neuronal migration ( J:107405 )

• at E16.5 the number of proliferating cells is decreased in the subventricular zone and at E18.5 no BrdU labelled cells are seen in the glomerular layer suggesting that migration of interneurons into the glomerular layer is impaired

craniofacial

abnormal olfactory sensory neuron morphology ( J:107405 )

• olfactory axons pass through the lamina cribosa by E12.5 but never reach the olfactory bulb and instead form a fibrocellular mass outside the olfactory bulb

• however, expression analysis indicates that differentiation of olfactory neurons is not significantly different from wild-type

respiratory system

abnormal olfactory sensory neuron morphology ( J:107405 )

• olfactory axons pass through the lamina cribosa by E12.5 but never reach the olfactory bulb and instead form a fibrocellular mass outside the olfactory bulb

• however, expression analysis indicates that differentiation of olfactory neurons is not significantly different from wild-type

taste/olfaction

abnormal olfactory sensory neuron morphology ( J:107405 )

• olfactory axons pass through the lamina cribosa by E12.5 but never reach the olfactory bulb and instead form a fibrocellular mass outside the olfactory bulb

• however, expression analysis indicates that differentiation of olfactory neurons is not significantly different from wild-type

growth/size/body

abnormal olfactory sensory neuron morphology ( J:107405 )

• olfactory axons pass through the lamina cribosa by E12.5 but never reach the olfactory bulb and instead form a fibrocellular mass outside the olfactory bulb

• however, expression analysis indicates that differentiation of olfactory neurons is not significantly different from wild-type

Genotype
MGI:3767818

cx2

• Allelic Composition: Fezf1^tm1Hibi/Fezf1^tm1Hibi; Fezf2^tm1Hibi/Fezf2^tm1Hibi
• Genetic Background: involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6

nervous system

abnormal diencephalon morphology ( J:119561 )

• at E18.5, the habenulo-intepeduncular tracts are small and abnormally located and the mammilothalamic tract is absent

• however, pretectum development is normal

• at E12.5, the prethalamus is absent and the caudal diencephalon is expanded

• at E10.5, regionalization of the diencephalons is defective

small thalamus ( J:119561 )

• the prethalamus is absent and the thalamus is reduced in size

abnormal hippocampus CA3 region morphology ( J:119561 )

• the CA3 region is strongly reduced

absent dentate gyrus ( J:119561 )

• the dentate gyrus is absent

abnormal neocortex morphology ( J:119561 )

• formation of the neocortex is impaired

small olfactory bulb ( J:119561 )

• the olfactory bulb is nearly completely lost

Genotype
MGI:3767819

cx3

• Allelic Composition: Fezf1^tm1Hibi/Fezf1^tm1Hibi; Fezf2^tm1Hibi/Fezf2⁺
• Genetic Background: involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6

nervous system

abnormal dentate gyrus morphology ( J:119561 )

• at E17.5

small olfactory bulb ( J:119561 )

• at E15.5