Phenotypes associated with this allele

• Allele Symbol: Prokr2^tm1Api
• Allele Name: targeted mutation 1, Shunichiro Matsumoto
• Allele ID: MGI:3623447
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prokr2^tm1Api/Prokr2^tm1Api	involves: C57BL/6 * CBA	MGI:3624437

Genotype
MGI:3624437

hm1

• Allelic Composition: Prokr2^tm1Api/Prokr2^tm1Api
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Prokr2^tm1Api/Prokr2^tm1Api mice, but not Prokr1^tm1Api/Prokr1^tm1Api mice, display olfactory bulb malformation

mortality/aging

neonatal lethality, incomplete penetrance ( J:107587 )

• about 50% die at an early neonatal stage

nervous system

abnormal olfactory bulb morphology ( J:107587 )

• the layered structure is absent or malformed and the glomerular layer is not detectable

abnormal olfactory bulb development ( J:107587 )

• at E16.5, E18.5 and P0, projection of the olfactory bulb from the telencephalon is very slight; however, at E14.5 gross morphology is similar to wild-type

• at E12.5 olfactory/vomeronasal axons fail to reach the forebrain and instead the terminals form a large spherical tangle

small olfactory bulb ( J:107587 )

• all homozygotes have small olfactory bulbs with a large space between the right and left bulbs

abnormal neuron morphology ( J:107587 )

• gonadotropin releasing hormone neurons are absent from the preoptic region and medial eminence of the hypothalamus

abnormal axon extension ( J:107587 )

• at E12.5 olfactory/vomeronasal axons fail to reach the forebrain and instead the terminals form a large spherical tangle

abnormal hypothalamus physiology ( J:107587 )

• hypothalmic expression of gonadotropin releasing hormone is significantly decreased

reproductive system

azoospermia ( J:107587 )

♂ • no sperm are seen in the lumen of the seminiferous tubules

arrest of male meiosis ( J:107587 )

♂ • spermatocytes and spermatogenic cells are present but spermatids are absent suggesting arrest at the pachytene stage

abnormal female reproductive system morphology ( J:107587 )

♀

absent corpus luteum ( J:107587 )

♀

impaired ovarian folliculogenesis ( J:107587 )

♀ • mostly underdeveloped follicles that appear to arrest at the preantral stage are present

decreased ovary weight ( J:107587 )

♀ • mean ovary weight is reduced to less than 0.1 mg compared to 3.4 mg in wild-type mice

ovary atrophy ( J:107587 )

♀ • atrophy, especially of the interstitium

uterus atrophy ( J:107587 )

♀ • atrophy, especially of the endometrium

vagina atrophy ( J:107587 )

♀ • atrophy of the epithelium

abnormal male reproductive system morphology ( J:107587 )

♂

abnormal seminiferous tubule morphology ( J:107587 )

♂ • the interstitium is sparse

small seminiferous tubules ( J:107587 )

♂

decreased Leydig cell number ( J:107587 )

♂ • few Leydig cells are seen and those that are present are smaller than normal

decreased testis weight ( J:107587 )

♂ • mean testis weight is reduced to 2.8 mg compared to 114.9 mg in wild-type mice

testicular atrophy ( J:107587 )

♂

homeostasis/metabolism

decreased circulating testosterone level ( J:107587 )

• in males

decreased follicle stimulating hormone level ( J:107587 )

• plasma levels are significantly decreased in males

endocrine/exocrine glands

abnormal mammary gland morphology ( J:107587 )

♀ • atrophy

absent corpus luteum ( J:107587 )

♀

impaired ovarian folliculogenesis ( J:107587 )

♀ • mostly underdeveloped follicles that appear to arrest at the preantral stage are present

decreased ovary weight ( J:107587 )

♀ • mean ovary weight is reduced to less than 0.1 mg compared to 3.4 mg in wild-type mice

ovary atrophy ( J:107587 )

♀ • atrophy, especially of the interstitium

abnormal seminiferous tubule morphology ( J:107587 )

♂ • the interstitium is sparse

small seminiferous tubules ( J:107587 )

♂

decreased Leydig cell number ( J:107587 )

♂ • few Leydig cells are seen and those that are present are smaller than normal

decreased testis weight ( J:107587 )

♂ • mean testis weight is reduced to 2.8 mg compared to 114.9 mg in wild-type mice

testicular atrophy ( J:107587 )

♂

integument

abnormal mammary gland morphology ( J:107587 )

♀ • atrophy

cellular

azoospermia ( J:107587 )

♂ • no sperm are seen in the lumen of the seminiferous tubules

arrest of male meiosis ( J:107587 )

♂ • spermatocytes and spermatogenic cells are present but spermatids are absent suggesting arrest at the pachytene stage

abnormal axon extension ( J:107587 )

• at E12.5 olfactory/vomeronasal axons fail to reach the forebrain and instead the terminals form a large spherical tangle