Phenotypes associated with this allele

• Allele Symbol: Hcls1^tm1Wtn
• Allele Name: targeted mutation 1, Takeshi Watanabe
• Allele ID: MGI:3623117
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hcls1^tm1Wtn/Hcls1^tm1Wtn	involves: 129P2/OlaHsd * C57BL/6	MGI:3625314
cx2	Hcls1^tm1Wtn/Hcls1^tm1Wtn Tg(TcraH-Y,TcrbH-Y)71Vbo/0	involves: 129P2/OlaHsd * C57BL/6J * DBA/2J	MGI:3625325

Genotype
MGI:3625314

hm1

• Allelic Composition: Hcls1^tm1Wtn/Hcls1^tm1Wtn
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased T cell proliferation ( J:28146 )

• purified T cells from null mice display reduced proliferation in culture compared to wild-type cells in response to anti-CD3, but proliferation of T cells is not reduced to same extent as proliferation of B cells in mutants

decreased B cell number ( J:28146 )

• in null mice, multivalent crosslinking of B cell surface antigen receptors does not significantly decrease the number of peritoneal B cells, as is seen in control mice

abnormal B cell physiology ( J:28146 )

• in null mice, multivalent crosslinking of B cell surface antigen receptors does not significantly decrease the number of peritoneal B cells, as is seen in control mice

immune system

decreased T cell proliferation ( J:28146 )

• purified T cells from null mice display reduced proliferation in culture compared to wild-type cells in response to anti-CD3, but proliferation of T cells is not reduced to same extent as proliferation of B cells in mutants

decreased B cell number ( J:28146 )

• in null mice, multivalent crosslinking of B cell surface antigen receptors does not significantly decrease the number of peritoneal B cells, as is seen in control mice

abnormal B cell physiology ( J:28146 )

• in null mice, multivalent crosslinking of B cell surface antigen receptors does not significantly decrease the number of peritoneal B cells, as is seen in control mice

abnormal humoral immune response ( J:28146 )

• antibody production to type II T-independent antigens is reduced in null mice while production of T-dependent antigen antibodies is not compared with levels in controls

cellular

decreased T cell proliferation ( J:28146 )

• purified T cells from null mice display reduced proliferation in culture compared to wild-type cells in response to anti-CD3, but proliferation of T cells is not reduced to same extent as proliferation of B cells in mutants

Genotype
MGI:3625325

cx2

• Allelic Composition: Hcls1^tm1Wtn/Hcls1^tm1Wtn; Tg(TcraH-Y,TcrbH-Y)71Vbo/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * DBA/2J

immune system

abnormal negative T cell selection ( J:28146 )

• in the thymus of transgenic male Hcls1-null mice, significant numbers of double positive thymocytes do not undergo negative selection and remain undeleted

decreased double-positive T cell number ( J:28146 )

• in Hcls1-expressing transgenic male mice, the population of double positive thymocytes is reduced to 10% of that in female ransgenic Hcls1-expressing animals

hematopoietic system

abnormal negative T cell selection ( J:28146 )

• in the thymus of transgenic male Hcls1-null mice, significant numbers of double positive thymocytes do not undergo negative selection and remain undeleted

decreased double-positive T cell number ( J:28146 )

• in Hcls1-expressing transgenic male mice, the population of double positive thymocytes is reduced to 10% of that in female ransgenic Hcls1-expressing animals