Phenotypes associated with this allele

• Allele Symbol: Ireb2^tm1Mwh
• Allele Name: targeted mutation 1, Matthias W Hentze
• Allele ID: MGI:3613022
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ireb2^tm1Mwh/Ireb2^tm1Mwh	involves: 129P2/OlaHsd * C57BL/6J	MGI:3613559

Genotype
MGI:3613559

hm1

• Allelic Composition: Ireb2^tm1Mwh/Ireb2^tm1Mwh
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal erythrocyte morphology ( J:105124 )

decreased hematocrit ( J:105124 )

• reduced hematocrit despite normal erythrocyte counts

decreased hemoglobin content ( J:105124 )

decreased mean corpuscular volume ( J:105124 )

microcytosis ( J:105124 )

decreased spleen iron level ( J:105124 )

• 40% decrease in nonheme iron content in the spleen

• however, no differences are seen in iron levels in the brain and mice do not display splenomegaly or erythroid hyperplasia

homeostasis/metabolism

abnormal iron homeostasis ( J:105124 )

• increase in ferritin H- and L-chain levels in the duodenum and liver and a decrease in ferritin and ferroportin protein expression, but not mRNA levels, in the spleen

increased intestinal iron level ( J:105124 )

• 80% increase in non-heme iron content in the duodenum, with iron deposits in the duodenal villi but not the crypts

• however no differences in serum iron levels, total iron binding capacity, serum transferring saturation, or rate of iron transport across the duodenal mucosa

decreased spleen iron level ( J:105124 )

• 40% decrease in nonheme iron content in the spleen

• however, no differences are seen in iron levels in the brain and mice do not display splenomegaly or erythroid hyperplasia

increased liver iron level ( J:105124 )

• 50% increase in non-heme iron content in the liver

immune system

decreased spleen iron level ( J:105124 )

• 40% decrease in nonheme iron content in the spleen

• however, no differences are seen in iron levels in the brain and mice do not display splenomegaly or erythroid hyperplasia

behavior/neurological

behavior/neurological phenotype ( J:105124 )

N • do not observe any overt signs of neuropathology such as tremor or posture abnormalities

abnormal locomotor behavior ( J:103414 )

• locomotion is mildly affected, however do not display the neurodegenerative phenotype seen in homozygous Ireb2^tm1Roua mice

digestive/alimentary system

increased intestinal iron level ( J:105124 )

• 80% increase in non-heme iron content in the duodenum, with iron deposits in the duodenal villi but not the crypts

• however no differences in serum iron levels, total iron binding capacity, serum transferring saturation, or rate of iron transport across the duodenal mucosa

liver/biliary system

increased liver iron level ( J:105124 )

• 50% increase in non-heme iron content in the liver